Anti-CD1c antibody [EPR23189-196] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Hodgkin's lymphoma. The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246520).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. (PMID : 15191950)The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246520).

• WB

Lab

Western blot - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab246520).

Western blot : Anti-CD1c antibody [EPR23189-196] ab246520 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. Samples were not boiled before loading onto the gel. A band was observed at 45 kDa in Wild-type Jurkat Not Boiled cell line with no signal observed at this size in CD1C knockout Jurkat Not Boiled cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CD1c antibody [EPR23189-196] (<a href='/ko/products/primary-antibodies/cd1c-antibody-epr23189-196-ab246520'>ab246520</a>) at 1/1000 dilution

Lane 1:

Wild-type Jurkat Not Boiled cell lysates at 20 µg

Lane 2:

Western blot - Human CD1C knockout Jurkat cell line (<a href='/ko/products/cell-lines/human-cd1c-knockout-jurkat-cell-line-ab273867'>ab273867</a>) at 20 µg

Lane 3:

MOLT-4 Not Boiled cell lysates at 20 µg

Lane 4:

HeLa Not Boiled cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 45 kDa

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