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AB212988

Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free

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Mouse Monoclonal CD2 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD2 protein.

대체 명칭 보기

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

3 이미지
Flow Cytometry - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Flow Cytometric Analysis of MOLT4 cells using ab193344 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193344).

Immunocytochemistry/ Immunofluorescence - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Immunofluorescence staining of MOLT-4 cells using ab193344 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193344).

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)
  • WB

Supplier Data

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

All lanes:

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (ab212988) at 2 µg/mL

All lanes:

Jurkat cell lysate

Predicted band size: 39 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

1E7E8.G4

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Cell preparation containing CD2 protein. The exact immunogen used to generate this antibody is proprietary information.

P06729

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대체품

Primary Antibodies

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PE/Cy5® Anti-CD2 antibody [RPA-2.10]

Flow Cytometry - PE/Cy5® Anti-CD2 antibody [RPA-2.10] (AB95556)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698)

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Primary Antibodies

AB119993

Anti-CD2 antibody [EPR3693]

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Western blot - Anti-CD2 antibody [EPR3693] (AB119993)

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Primary Antibodies

AB131276

Anti-CD2 antibody [EPR6451]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] (AB131276)

  • 0
  • 0
Primary Antibodies

AB219411

Anti-CD2 antibody [EPR21825]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

  • 0
  • 0
Primary Antibodies

AB247951

Anti-CD2 antibody [EPR3693] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CD2 antibody [EPR3693] - BSA and Azide free (AB247951)

  • 0
  • 0
Primary Antibodies

AB248400

Anti-CD2 antibody [EPR6451] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (AB248400)

  • 0
  • 0
Primary Antibodies

AB231059

Anti-CD2 antibody [EPR21825] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (AB231059)

  • 0
  • 0

보완 제품

Primary Antibodies

AB308459

Anti-CD58 antibody [EPR27435-85]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD58 antibody [EPR27435-85] (AB308459)

  • 0
  • 0
Primary Antibodies

AB281586

Anti-CD45 antibody [RM1007]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)

  • 5
  • 3
Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

  • 5
  • 2
Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

  • 4
  • 10
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

ab212988 is a carrier free version of ab193344.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.
Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
See full target information CD2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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