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AB244336

Anti-CD20 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal CD20 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MS4A1 aa 200-300.

대체 명칭 보기

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

8 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human gastrointestinal tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Low expression as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody (AB244336)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody (AB244336)

PFA fixed, Triton X-100 permeabilized REH (Human cell line) cells labeling CD20 using ab244336 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human testis tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-CD20 antibody (AB244336)
  • WB

Unknown

Western blot - Anti-CD20 antibody (AB244336)

All lanes:

Western blot - Anti-CD20 antibody (ab244336) at 0.4 µg/mL

Lane 1:

Daudi cells

Lane 2:

U-2 OS cells

Predicted band size: 33 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MS4A1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P11836

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대체품

Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
Primary Antibodies

AB78237

Anti-CD20 antibody [EP459Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD20 antibody [EP459Y] (AB78237)

  • 4
  • 7
Primary Antibodies

AB166865

Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (AB166865)

  • 0
  • 0
Primary Antibodies

AB198941

Alexa Fluor® 488 Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD20 antibody [EP459Y] (AB198941)

  • 4
  • 3
Primary Antibodies

AB198943

Alexa Fluor® 647 Anti-CD20 antibody [EP459Y]

Recombinant|Advanced Validation|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD20 antibody [EP459Y] (AB198943)

  • 4
  • 1
Primary Antibodies

AB236434

Anti-CD20 antibody [SP32] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] - BSA and Azide free (AB236434)

  • 5
  • 3

보완 제품

Assay Kits

AB285273

Human CD20 ELISA Kit

Sandwich ELISA - Human CD20 ELISA Kit (AB285273)

  • 0
  • 0
Primary Antibodies

AB79414

Anti-CD79a antibody [EP3618]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB134114

Anti-CD19 antibody [EPR5906]

KO Validated|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD19 antibody [EPR5906] (AB134114)

  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 9: PubMed39088302

2024

Human gut microbiota-reactive DP8α Tregs prevent acute graft-versus-host disease in a CD73-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuelle Godefroy,Patrice Chevallier,Fabienne Haspot,Caroline Vignes,Véronique Daguin,Sylvia Lambot,Margaux Verdon,Margaux De Seilhac,Valentin Letailleur,Anne Jarry,Annabelle Pédron,Thierry Guillaume,Pierre Peterlin,Alice Garnier,Marie-Anne Vibet,Maxence Mougon,Amandine Le Bourgeois,Maxime Jullien,Francine Jotereau,Frédéric Altare

Cell death & disease 14:83 PubMed36739418

2023

VHL-HIF-2α axis-induced SEMA6A upregulation stabilized β-catenin to drive clear cell renal cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ji,Yuxin Xu,Mengru Xie,Xingbei He,Dexu Ren,Teng Qiu,Wenwen Liu,Zefeng Chen,Wen Shi,Zhen Zhang,Xiujun Wang,Weiling Wang,Jinming Ma,Qilan Qian,Aixin Jing,Xinhui Ma,Jingting Qin,Yuanyuan Ding,Ting Geng,Jiayan Yang,Zhichao Sun,Wei Liu,Shunfang Liu,Bin Liu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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