AB215866

Anti-CD20 antibody [IGEL/773]

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(2 제품이 사용된 논문 )

Mouse Monoclonal CD20 antibody. Suitable for ICC, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MS4A1.

대체 명칭 보기

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

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Supplier Data

Immunocytochemistry - Anti-CD20 antibody [IGEL/773] (AB215866)

Immunocytochemistry immunoflourescence image staining Raji cells (human Burkitt's lymphoma cell line) stained with ab215866 (green), nuclear counterstaining with redDot (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] (AB215866)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] (AB215866)

Immunohistochemical analysis of paraffin embedded, formalin fixed Human tonsil tissue labeling CD20 using ab215866 at 1 μg/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] (AB215866)

Immunohistochemical analysis of paraffin embedded, formalin fixed Human lymphoma tissue labeling CD20 using ab215866 at 1 μg/mL dilution.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

IGEL/773

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MS4A1.

P11836

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대체품

Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

Primary Antibodies

AB78237

Anti-CD20 antibody [EP459Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD20 antibody [EP459Y] (AB78237)

Primary Antibodies

AB166865

Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (AB166865)

Primary Antibodies

AB198941

Alexa Fluor® 488 Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD20 antibody [EP459Y] (AB198941)

Primary Antibodies

AB198943

Alexa Fluor® 647 Anti-CD20 antibody [EP459Y]

Recombinant|Advanced Validation|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD20 antibody [EP459Y] (AB198943)

Primary Antibodies

AB236434

Anti-CD20 antibody [SP32] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] - BSA and Azide free (AB236434)

보완 제품

Assay Kits

AB285273

Human CD20 ELISA Kit

Sandwich ELISA - Human CD20 ELISA Kit (AB285273)

Primary Antibodies

AB196515

Alexa Fluor® 647 Anti-CD19 antibody [EPR5906]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD19 antibody [EPR5906] (AB196515)

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB20181

Anti-HLA-DR antibody [TAL 1B5]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (AB20181)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from bioreactor concentrate.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer science 114:1846-1858 PubMed36748311

2023

Prognostic relevance of tumor-resident memory T cells in metastatic lymph nodes of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Seiji Natsuki,Hiroaki Tanaka,Masaki Nishiyama,Takuya Mori,Sota Deguchi,Yuichiro Miki,Mami Yoshii,Tatsuro Tamura,Takahiro Toyokawa,Shigeru Lee,Kiyoshi Maeda

Archives of pathology & laboratory medicine 147:167-176 PubMed35687787

2022

Plasma Galectin-9 Is a Useful Biomarker for Predicting Renal Function in Patients Undergoing Native Kidney Biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Tsun Tsai,Ruey-Bing Yang,Shuo-Ming Ou,Wei-Cheng Tseng,Kuo-Hua Lee,Chih-Yu Yang,Fu-Pang Chang,Der-Cherng Tarng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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