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AB8237

Anti-CD20 antibody [MEM-97]

4

(1 리뷰)

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(3 제품이 사용된 논문 )

Mouse Monoclonal CD20 antibody. Suitable for IP, Flow Cyt and reacts with Human, Pig, Dog samples. Cited in 3 publications.
4 이미지
Flow Cytometry - Anti-CD20 antibody [MEM-97] (AB8237)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD20 antibody [MEM-97] (AB8237)

Double Immunostaining of CD20 and CD79a in human, canine and porcine B lymphocytes using ab8237 and ab76908 by Flow Cytometery.

Flow Cytometry - Anti-CD20 antibody [MEM-97] (AB8237)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [MEM-97] (AB8237)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab8237 (concentration in sample 1.6 μg/ml) GAM APC.

Flow Cytometry - Anti-CD20 antibody [MEM-97] (AB8237)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [MEM-97] (AB8237)

Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab8237 (concentration in sample 1.6 μg/ml) GAM APC.

Immunoprecipitation - Anti-CD20 antibody [MEM-97] (AB8237)
  • IP

Unknown

Immunoprecipitation - Anti-CD20 antibody [MEM-97] (AB8237)

Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1 : original lysate of RAJI cells Lane 2 : immunoprecipitate by Isotypic mouse IgG1 control Lane 3 : original lysate of RAJI cells Lane 4 : immunoprecipitate by anti-CD20 (MEM-97, ab 8237)

All lanes:

Immunoprecipitation - Anti-CD20 antibody [MEM-97] (ab8237)

Predicted band size: 33 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-97

Isotype

IgG1

Light chain 타입

unknown

Carrier free

No

Reacts with

Dog, Human, Pig

Applications

IP, Flow Cyt

applications

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Pig": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

제품 세부 정보

This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified from TCS
보관 버퍼
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1

대체 명칭 보기

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 21:254-264 PubMed27860183

2016

MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2.

Applications

Flow Cyt

Species

Human

Guo-Ping Zhang,Jing Zhang,Chao-Hua Zhu,Lei Lin,Jian Wang,Hai-Jing Zhang,Jun Li,Xiao-Guang Yu,Zhen-Shuan Zhao,Wei Dong,Guo-Bin Liu

Oncotarget 2:59-68 PubMed21378416

2011

A robust approach to enhance tumor-selective accumulation of nanoparticles.

Applications

Unspecified application

Species

Mouse

Yuan Qiao,Xin Huang,Sridhar Nimmagadda,Renyuan Bai,Verena Staedtke,Catherine A Foss,Ian Cheong,Matthias Holdhoff,Yoshinori Kato,Martin G Pomper,Gregory J Riggins,Kenneth W Kinzler,Luis A Diaz,Bert Vogelstein,Shibin Zhou

Journal of immunology (Baltimore, Md. : 1950) 157:2939-46 PubMed8816400

1996

Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY.

Applications

Unspecified application

Species

Unspecified reactive species

J Szöllósi,V Horejsí,L Bene,P Angelisová,S Damjanovich
제품이 사용된 논문 모두 보기

Product promise

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