Anti-CD20 antibody [PDR78] - BSA and Azide free

• Flow Cyt

Lab

Flow Cytometry - Anti-CD20 antibody [PDR78] - BSA and Azide free (AB255940)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254189).

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab254189 (right) or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab254189 or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (1x 10⁶ in 100 µl at 1.0 μg/ml (1/500)) for 30min on ice. The cells were simultaneously stained with CD19.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

• Flow Cyt

Unknown

Flow Cytometry - Anti-CD20 antibody [PDR78] - BSA and Azide free (AB255940)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD20 with ab254189 at 1.11μg/mL (0.1ug) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with mouse IgG (Left) or ab254189 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254189).