AB244564

Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free

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Rat Monoclonal CD200 / OX2 antibody. Carrier free. Suitable for Flow Cyt, ELISA, IHC-FoFr, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

대체 명칭 보기

CD200, Mox2, Cd200, OX-2 membrane glycoprotein, MRC OX-2 antigen

1 이미지

Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (AB244564)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (AB244564)

This data was developed using the same antibody clone in a different buffer formulation (ab33734)

C57BL/6 mouse splenocytes stained with ab33734 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33734) or Rat IgG2aκ (ab18450) (1x¹⁰⁶ in 100 μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ^® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD19 antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

MRC OX90

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-Fr, ELISA, IHC-FoFr, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

ab244564 is the carrier-free version of ab33734.

Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.

Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity).

See full target information Cd200

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Immunology 102:173-9 PubMed11260322

2001

The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.

Applications

Unspecified application

Species

Unspecified reactive species

G J Wright,M Jones,M J Puklavec,M H Brown,A N Barclay

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free

Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (AB244564)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise