AB227787

Anti-CD22 antibody - C-terminal

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal CD22 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody - C-terminal (AB227787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody - C-terminal (AB227787)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD22 with ab227787 (1/200 dilution) in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

대체품

Primary Antibodies

AB227645

Anti-CD22 antibody [SP104] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

0
0

Primary Antibodies

AB32355

Anti-CD22 (phospho Y842) antibody [Y507]

RabMAb|Recombinant

Western blot - Anti-CD22 (phospho Y842) antibody [Y507] (AB32355)

0
0

Primary Antibodies

AB33859

Anti-CD22 antibody [EP498Y]

RabMAb|Recombinant

Western blot - Anti-CD22 antibody [EP498Y] (AB33859)

0
0

Primary Antibodies

AB207727

Anti-CD22 antibody [EPR20061]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] (AB207727)

4
2

Primary Antibodies

AB240404

Anti-CD22 antibody [SP104] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - BSA and Azide free (AB240404)

0
0

Primary Antibodies

AB246334

Anti-CD22 antibody [EPR20061] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] - BSA and Azide free (AB246334)

0
0

Primary Antibodies

AB32123

Anti-CD22 (phospho Y822) antibody [Y506]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

3
1

Primary Antibodies

AB32040

Anti-CD22 (phospho Y807) antibody [Y505]

RabMAb|Recombinant

Western blot - Anti-CD22 (phospho Y807) antibody [Y505] (AB32040)

0
0

보완 제품

Primary Antibodies

AB134114

Anti-CD19 antibody [EPR5906]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD19 antibody [EPR5906] (AB134114)

4
5

Primary Antibodies

AB201811

Anti-PD1 antibody [NAT105] - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811)

0
0

Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

5
12

Primary Antibodies

AB219650

Anti-PRAME antibody [EPR20330]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAME antibody [EPR20330] (AB219650)

0
0

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).

See full target information CD22

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:3220 PubMed38622115

2024

Reciprocal antagonism of PIN1-APC/C governs mitotic protein stability and cell cycle entry.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhong Ke,Fabin Dang,Lin Wang,Jia-Yun Chen,Mandar T Naik,Wenxue Li,Abhishek Thavamani,Nami Kim,Nandita M Naik,Huaxiu Sui,Wei Tang,Chenxi Qiu,Kazuhiro Koikawa,Felipe Batalini,Emily Stern Gatof,Daniela Arango Isaza,Jaymin M Patel,Xiaodong Wang,John G Clohessy,Yujing J Heng,Galit Lahav,Yansheng Liu,Nathanael S Gray,Xiao Zhen Zhou,Wenyi Wei,Gerburg M Wulf,Kun Ping Lu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com