AB32123

Anti-CD22 (phospho Y822) antibody [Y506]

Name: Anti-CD22 (phospho Y822) antibody [Y506]
Brand: Abcam Limited
SKU: AB32123
Rating: 3 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CD22 phospho Y822 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

3 이미지

Flow Cytometry (Intracellular) - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Flow cytometric analysis of Ramos cells treated with 1mM pervanadate for 30min labelling CD22 (phospho Y822) with ab32123 at 1/1000 dilutioncompared with an isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were fixed with 100μl 4% PFA for 10 min at RT, then permeabilized with 100μl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab32123 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.

Immunocytochemistry/ Immunofluorescence - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Ramos cells with or without treatment labelling CD22 (phospho Y822) with ab32123 at 1/200 dilution followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (Green). Confocal images showing no staining in Ramos cells and strong membranous staining in Ramos treated with pervanadate (1mM 30min) but weak membranous staining in Ramos treated with pervanadate (1mM 30min) and phosphatase.
ab7291 Anti-beta Tubulin, used as a counterstain at 1/1000 dilution, was co-incubated with ab32123 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Mouse secondary (ab150120) at 1/1000 dilution at RT for 45 min (Magenta). Nucleus were visualized using DAPI.

Lab

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.

All lanes:

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

Lane 1:

Untreated Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Ramos treated with 1mM pervanadate for 30min whole cell lysate at 15 µg

Lane 3:

Ramos treated with 1mM pervanadate for 30min whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 150 kDa

true

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y506

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" } } }

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제품 세부 정보

The production method for this product has been changed from hybridoma to recombinant on 23rd April 2026

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).

See full target information CD22 pY822

대체 명칭 보기

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature immunology 24:1762-1777 PubMed37653247

2023

Molecular basis for potent B cell responses to antigen displayed on particles of viral size.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy F Brooks,Julianne Riggs,James L Mueller,Raisa Mathenge,Wei-Yun Wholey,Alexander R Meyer,Sekou-Tidiane Yoda,Vivasvan S Vykunta,Hailyn V Nielsen,Wei Cheng,Julie Zikherman

Proceedings of the National Academy of Sciences of 112:E6515-24 PubMed26561584

2015

An extracatalytic function of CD45 in B cells is mediated by CD22.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Coughlin,Mark Noviski,James L Mueller,Ammarina Chuwonpad,William C Raschke,Arthur Weiss,Julie Zikherman

Blood 116:1698-704 PubMed20516366

2010

IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes.

Applications

Flow Cyt

Species

Human

Jean-François Séïté,Divi Cornec,Yves Renaudineau,Pierre Youinou,Rizgar A Mageed,Sophie Hillion

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD22 (phospho Y822) antibody [Y506]

Flow Cytometry (Intracellular) - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Immunocytochemistry/ Immunofluorescence - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise