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AB273676

Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CD226 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (Detector) (AB273676)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD226 antibody [EPR23641-137] - BSA and Azide free (Detector) (AB273676)

Example of Human CD226 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23641-137

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB282303

Human CD226 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD226 ELISA Kit (AB282303)

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Sample Prep & Detection Kits

AB201805

DyLight® 755 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 755 Conjugation Kit (Fast) - Lightning-Link® (AB201805)

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Sample Prep & Detection Kits

AB269892

Latex Conjugation Kit - 400nm Blue

Functional Studies - Latex Conjugation Kit - 400nm Blue (AB269892)

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Primary Antibodies

AB272282

Anti-Alcam antibody [EPR22341-182] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Alcam antibody [EPR22341-182] - BSA and Azide free (Detector) (AB272282)

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Primary Antibodies

AB244746

Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB244746)

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Primary Antibodies

AB281048

Anti-Hsp27 antibody [EPR23938-91] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Hsp27 antibody [EPR23938-91] - BSA and Azide free (Detector) (AB281048)

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Primary Antibodies

AB242556

Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] - BSA and Azide free (Capture) (AB242556)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab273676 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD226. The recommended pair for sandwich ELISA is:
Capture: ab273677, Human CD226 Capture Antibody (unconjugated)
Detector: ab273676, Human CD226 Detector Antibody (unconjugated)
The reference range value is 7.8 - 500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
See full target information CD226

대체 명칭 보기

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1
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Product promise

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