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AB254162

Anti-CD23 antibody [BU38]

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(2 제품이 사용된 논문 )

Mouse Recombinant Monoclonal CD23 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 2 publications.
2 이미지
Flow Cytometry - Anti-CD23 antibody [BU38] (AB254162)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD23 antibody [BU38] (AB254162)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD23 with ab254162 at 11.14μg/ml, compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254162 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [BU38] (AB254162)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [BU38] (AB254162)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD23 with ab254162 at 1.114μg/ml, followed by ready to use secondary. Positive staining on germinal center in the human tonsil is observed. The section was incubated with ab254162 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-CD23 antibody [BU38] - BSA and Azide free

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

BU38

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB16702

Anti-CD23 antibody [SP23]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

  • 0
  • 0
Primary Antibodies

AB135386

Anti-CD23 antibody [SP163]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] (AB135386)

  • 0
  • 0
Primary Antibodies

AB247568

Anti-CD23 antibody [EPR3617] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD23 antibody [EPR3617] - BSA and Azide free (AB247568)

  • 0
  • 0
Primary Antibodies

AB245732

Anti-CD23 antibody [SP163] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732)

  • 0
  • 0
Primary Antibodies

AB92495

Anti-CD23 antibody [EPR3617]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (AB92495)

  • 0
  • 0

보완 제품

Assay Kits

AB272468

Human CD23 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD23 ELISA Kit (AB272468)

  • 0
  • 0
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB75985

Anti-CD21 antibody [EP3093]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] (AB75985)

  • 4
  • 6
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 13
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "11.14 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1.114 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.
Biological function summary

CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

Pathways

CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells (PubMed : 2167225). On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed : 7544003).
See full target information FCER2

대체 명칭 보기

CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal for immunotherapy of cancer 13: PubMed39824529

2025

Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Lahusen,Nora Minhöfer,Kim-André Lohse,Christine Blechner,Jessica Lindenmayer,Tim Eiseler,Anton Wellstein,Alexander Kleger,Thomas Seufferlein,Sabine Windhorst,Yuan-Na Lin

BMC immunology 24:4 PubMed36650424

2023

PU.1-CD23 signaling mediates pulmonary innate immunity against Aspergillus fumigatus infection by driving inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Ming Zhang,Jiayong Qiu,Chenyang Liu,Yao Lou,Tongsheng Wang,Yingmin Zhang,Yimin Mao
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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