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AB233809

Anti-CD272/BTLA antibody [4B12A7]

5

(1 리뷰)

|

(1 출판물)

Mouse Monoclonal CD272/BTLA antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BTLA aa 150-300.

대체 명칭 보기

CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA

3 이미지
Flow Cytometry - Anti-CD272/BTLA antibody [4B12A7] (AB233809)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling CD272/BTLA with ab233809 at 1/200 dilution (green) compared with a negative control (red).

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)
  • WB

Supplier Data

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

All lanes:

Western blot - Anti-CD272/BTLA antibody [4B12A7] (ab233809) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

CD272/BTLA (aa 179-289)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 33 kDa

false

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)
  • WB

Ap

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Expected recombinant MW is 38 kDa.

All lanes:

Western blot - Anti-CD272/BTLA antibody [4B12A7] (ab233809) at 1/500 dilution

All lanes:

Recombniant human CD272/BTLA (aa 179-289) protein

Predicted band size: 33 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

4B12A7

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human BTLA aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6A9

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대체품

Primary Antibodies

AB181406

Anti-CD272/BTLA antibody

Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody (AB181406)

  • 0
  • 0
Primary Antibodies

AB216505

Anti-CD272/BTLA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody (AB216505)

  • 3
  • 1
Primary Antibodies

AB224676

Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [EPR20539] - BSA and Azide free (AB224676)

  • 0
  • 0
Primary Antibodies

AB233733

Anti-CD272/BTLA antibody [7B8B4]

Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [7B8B4] (AB233733)

  • 0
  • 0
Primary Antibodies

AB96560

Anti-CD272/BTLA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody (AB96560)

  • 0
  • 0
Primary Antibodies

AB212089

Anti-CD272/BTLA antibody [EPR20539]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [EPR20539] (AB212089)

  • 0
  • 0

보완 제품

Primary Antibodies

AB201811

Anti-PD1 antibody [NAT105] - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811)

  • 0
  • 0
Primary Antibodies

AB131254

Anti-CD27 antibody [EPR8569]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8569] (AB131254)

  • 5
  • 2
Primary Antibodies

AB246518

Anti-CXCL13 antibody [EPR23400-92]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

  • 5
  • 1
Primary Antibodies

AB241332

Anti-TIM 3 antibody [EPR22241]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [EPR22241] (AB241332)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from tissue culture supernatant.
보관 버퍼
Preservative: 0.05% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD272 also known as BTLA (B and T Lymphocyte Attenuator) is an immunoregulatory protein with a mass approximately 34 kDa. This target is part of the CD28 immunoglobulin superfamily and is expressed on the surfaces of various immune cells including T cells B cells and dendritic cells. BTLA/CD272 serves as a co-inhibitory molecule important in maintaining immune homeostasis. Its expression is influenced by factors present in the microenvironment of immune tissues.
Biological function summary

BTLA functions as a negative regulator of immune responses by delivering inhibitory signals to immune cells upon binding to its ligand HVEM (Herpesvirus entry mediator). It forms a part of an immunological checkpoint complex that modulates lymphocyte activity to prevent overactivation and autoimmunity. This mechanism helps maintain a balance between immune defense and tolerance contributing to self-tolerance.

Pathways

The BTLA/CD272 protein engages with pathways involved in immune checkpoints and T-cell receptor signaling. It interacts with proteins like HVEM and collaborates with receptors such as PD-1 and CTLA-4. These interactions play a role in the downregulation of T-cell responses which is essential in various immune processes. The signaling pathways influenced by BTLA/CD272 are integral in moderating immune responses cooperation and adaptation.

BTLA is associated with autoimmune diseases and cancer. In autoimmune disorders an altered expression or function of BTLA can lead to dysregulated immune responses causing tissue damage. In cancer the protein may contribute to immune evasion by tumors aiding tumor growth and progression. The interplay of BTLA with proteins such as PD-1 makes it a potential target for immunotherapy strategies in oncology and autoimmunity treatments.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 12796776, PubMed : 14652006, PubMed : 15568026, PubMed : 18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed : 19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed : 19915044).
See full target information BTLA
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular cell 82:2779-2796.e10 PubMed35675814

2022

AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Cho,Elizabeth T Abshire,Maximilian W Popp,Christoph Pröschel,Joshua L Schwartz,Gene W Yeo,Lynne E Maquat
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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