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AB243883

Anti-CD276 antibody [BLR026F] - BSA free

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CD276 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD276.

대체 명칭 보기

CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3

4 이미지
Immunocytochemistry - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)
  • ICC

Unknown

Immunocytochemistry - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)

Formalin-fixed, paraffin-embedded A172 cells labeling CD276 using ab243883 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CD276 using ab243883 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Immunoprecipitation - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)
  • IP

Unknown

Immunoprecipitation - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)

CD276 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243883 at 3 µl per reaction. Western blot was performed on the immunoprecipitate using ab243883 at 1/1000 dilution. Lane 1 : ab243883 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)

Predicted band size: 57 kDa

false

Western blot - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)
  • WB

Unknown

Western blot - Anti-CD276 antibody [BLR026F] - BSA free (AB243883)

Western blot analysis using ab243883 at 1/1000 dilution.

Lane 1 : MCF7 whole cell lysate.

Lane 2 : A172 whole cell lysate.

Lane3 : U-251 MG whole cell lysate.

Lane 4 : OVCAR-3 whole cell lysate.

Lane 5 : HeLa whole cell lysate.

Lane 6 : HEK-293T whole cell lysate.

Lane 7 : HCT 116 whole cell lysate.

Lane 8 : RPMI 8226 whole cell lysate.

Lane 9 : MJ whole cell lysate.

Lane 10 : Ramos whole cell lysate.

Lane 11 : Jurkat whole cell lysate.

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence.

All lanes:

Western blot - Anti-CD276 antibody [BLR026F] - BSA free (ab243883)

Predicted band size: 57 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR026F

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human CD276. The exact immunogen used to generate this antibody is proprietary information.

Q5ZPR3

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대체품

Primary Antibodies

AB218280

Alexa Fluor® 488 Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD276 antibody [EPNCIR122] (AB218280)

  • 0
  • 0
Primary Antibodies

AB218283

Alexa Fluor® 555 Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CD276 antibody [EPNCIR122] (AB218283)

  • 0
  • 0
Primary Antibodies

AB134161

Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-CD276 antibody [EPNCIR122] (AB134161)

  • 0
  • 0
Primary Antibodies

AB219648

Anti-CD276 antibody [EPR20115]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [EPR20115] (AB219648)

  • 4
  • 1
Primary Antibodies

AB256585

Anti-CD276 antibody [EPNCIR122] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD276 antibody [EPNCIR122] - BSA and Azide free (AB256585)

  • 0
  • 0
Primary Antibodies

AB227670

Anti-CD276 antibody [SP206]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206] (AB227670)

  • 5
  • 1
Primary Antibodies

AB227577

Anti-CD276 antibody [EPR20115] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [EPR20115] - BSA and Azide free (AB227577)

  • 0
  • 1
Primary Antibodies

AB209895

Anti-CD276 antibody [EPNCIR122] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD276 antibody [EPNCIR122] - Low endotoxin, Azide free (AB209895)

  • 0
  • 0

보완 제품

Assay Kits

AB221831

Human CD276 ELISA Kit

Recombinant

ELISA - Human CD276 ELISA Kit (AB221831)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB38449

Anti-AKT (phospho T308) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT (phospho T308) antibody (AB38449)

  • 3
  • 5
Primary Antibodies

AB179463

Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (AB179463)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 3μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.
Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
See full target information CD276
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular psychiatry 30:5680-5693 PubMed40962831

2025

Mutations of schizophrenia risk gene SETD1A dysregulate synaptic function in human neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Su,Hanwen Zhang,Yan Hong,Qian Yang,Le Wang,Tiffany Le,Jiayi Liu,Lasya Cheruvu,Emily Labour,Siwei Zhang,Karla Mendez-Maldonado,Anat Kreimer,Hongjun Song,Guo-Li Ming,Jubao Duan,Zhiping P Pang

Translational cancer research 13:833-846 PubMed38482406

2024

B7-H3 promotes proliferation and migration of lung cancer cells by modulating PI3K/AKT pathway via ENO1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xianan Wu,Congcong Ding,Yingqi Liu,Ke Dong,Huizhong Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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