AB25109

Anti-CD3 antibody [FN-18]

Lab Essentials
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(2 제품이 사용된 논문 )

Mouse Monoclonal CD3d antibody. Suitable for IHC-Fr and reacts with Rhesus monkey samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

대체 명칭 보기

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 이미지

Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [FN-18] (AB25109)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [FN-18] (AB25109)

Immunohistochemistry (Frozen sections) analysis of lymph node tissue from Rhesus macaque labeling CD3 with ab25109 at 1/100 dilution. Tissue was fixed in acetone, blocked using 10% goat serum/PBS for 15 min at 21°C, then incubated with Anti-CD3 antibody [FN-18] (ab25109) at 1/100 dilution for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse polyclonal was used as the secondary antibody.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

FN-18

Isotype

IgG1

Carrier free

No

Reacts with

Rhesus monkey

Applications

IHC-Fr

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

4
46

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

4
80

Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

5
1

Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

0
0

Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

5
3

Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

4
10

Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

4
2

보완 제품

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

4
80

Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

0
0

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

5
21

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" }, "Rhesus monkey": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 관련 사항

Ion exchange chromatography

보관 버퍼

Constituents: PBS, 4.275% Trehalose

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of leukocyte biology 96:957-67 PubMed25114162

2014

HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

James T Frencher,Hongbo Shen,Lin Yan,Jessica O Wilson,Nancy E Freitag,Alicia N Rizzo,Crystal Y Chen,Zheng W Chen

Journal of immunology (Baltimore, Md. : 1950) 179:3325-31 PubMed17709549

2007

"Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics.

Applications

Flow Cyt

Species

Cynomolgus monkey

Richard Stebbings,Lucy Findlay,Cherry Edwards,David Eastwood,Chris Bird,David North,Yogesh Mistry,Paula Dilger,Emily Liefooghe,Isabelle Cludts,Bernard Fox,Gill Tarrant,Jane Robinson,Tony Meager,Carl Dolman,Susan J Thorpe,Adrian Bristow,Meenu Wadhwa,Robin Thorpe,Stephen Poole

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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