AB243873

Anti-CD3 epsilon antibody [BL-298-5D12]

Recombinant
Lab Essentials
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CD3E antibody. Suitable for IHC-P, IP, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CD3E aa 150 to C-terminus.

대체 명칭 보기

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human lung carcinoma stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining.

Flow Cytometry - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Flow cytometric analysis of Jurkat cells incubated with 1 μL ab243873 (red shaded) or isotype control (unshaded). Cells were fixed with 4% formaldehyde, and permeabilized with 90% methanol. DyLight^® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human breast carcinoma stained for CD3E using ab243873 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3E using ab243873 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human appendix stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A DyLight^® 594-conjugated goat anti-rabbit IgG was used as the secondary.

Supplier Data

Immunoprecipitation - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

CD3E was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243873 at 20 μL per reaction. Western blot was performed on the immunoprecipitate using ab243873 at 1/1000 dilution.

Detection : Chemiluminescence.

All lanes:

Immunoprecipitation - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873) at 1/1000 dilution

Lane 1:

ab243873 IP in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

Contol IgG in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

Predicted band size: 23 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Detection : chemiluminescence

All lanes:

Western blot - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 50 µg

Lane 3:

SK-MEL-28 whole cell lysate at 50 µg

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 23 kDa

false

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-298-5D12

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human CD3E aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07766

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20μl/mg lysate.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형

Liquid

Purification 관련 사항

Recombinant antibody was purified from cell culture supernatant.

보관 버퍼

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099).

See full target information CD3E

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 30:456 PubMed40762017

2025

Acute myeloid leukemia with plasmacytoid dendritic cell proliferation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Pei,Yi Zhang,Han Xu,Pei Pei,Zhengyang Zhang,Hongxia Wang,Bei Zhang,Junjun Bai,Yingxin Zhao,Jingjing Gu,Zhiyu Fang,Miaomiao Liu,Qinglin Song

Regenerative biomaterials 11:rbad116 PubMed38333727

2024

Harnessing cytokine-induced killer cells to accelerate diabetic wound healing: an approach to regulating post-traumatic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yixi Yang,Cheng Zhang,Yuan Jiang,Yijun He,Jiawei Cai,Lin Liang,Zhaohuan Chen,Sicheng Pan,Chu Hua,Keke Wu,Le Wang,Zhiyong Zhang

Nature communications 14:5147 PubMed37620345

2023

RNAPII-dependent ATM signaling at collisions with replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Einig,Chao Jin,Valentina Andrioletti,Boris Macek,Nikita Popov

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD3 epsilon antibody [BL-298-5D12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Flow Cytometry - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Immunoprecipitation - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Western blot - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise