Anti-CD30 antibody [mCD30.1] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [mCD30.1] - BSA and Azide free (AB324908)

This data was developed using ab324907, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells treated with 1ug/ml of immobilized anti-CD3 and 10ug/ml anti-CD28 for 3 days (Green and Black) / Untreated control (Magenta and Grey) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Magenta and Green) compared with a Armenian Hamster monoclonal IgG (Black and Grey) isotype control.

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable CD8 positive cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [mCD30.1] - BSA and Azide free (AB324908)

This data was developed using ab324907, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells treated with 2.5ug/ml Concanavalin A for 72 hours (Green and Black) / Untreated control (Magenta and Grey) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Magenta and Green) compared with a Armenian Hamster monoclonal IgG (Black and Grey) isotype control.

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable CD8 positive cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [mCD30.1] - BSA and Azide free (AB324908)

This data was developed using ab324907, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / YAC-1 (mouse Moloney leukemia virus induced lymphoma lymphoblast, Right) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Red) compared with a Armenian Hamster monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : NIH/3T3.

• Dot

Supplier Data

Dot Blot - Anti-CD30 antibody [mCD30.1] - BSA and Azide free (AB324908)

This data was developed using ab324907, the same antibody clone in a different buffer formulation.

Dot blot analysis of CD30 using ab324907 at 1 : 1000 (0.998 ug/ml) followed by a Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1 : 2000 dilution.

Lane1 : Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein

Exposure time : 10 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CD30 antibody [mCD30.1] (<a href='/ko/products/primary-antibodies/cd30-antibody-mcd301-ab324907'>ab324907</a>) at 1/1000 dilution

All lanes:

Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein

Secondary

All lanes:

Dot Blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/rabbit-armenian-hamster-igg-h-l-hrp-ab5745'>ab5745</a>) at 1/2000 dilution

false

Exposure time: 10s