AB11032

Anti-CD33 antibody [6C5/2]

Name: Anti-CD33 antibody [6C5/2]
Brand: Abcam Limited
SKU: AB11032
Rating: 3 (3 reviews)

BOND RX™ Validated
각 태그의 뜻

(3 리뷰들)

(11 제품이 사용된 논문 )

Mouse Monoclonal CD33 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD33.

대체 명칭 보기

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (AB11032)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (AB11032)

IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

6C5/2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CD33.

P20138

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).

See full target information CD33

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of gastrointestinal oncology 16:1248-1257 PubMed40672064

2025

Peripheral blood neutrophil-to-lymphocyte ratio as a prognostic marker and its association with the tumor-immune microenvironment in pancreatic cancer: a retrospective cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Jiayi Wang,Yuxuan Li,Wenna Jiang,Duo Zuo,Xiuse Zhang,Jiawei Xiao,Kentaro Inamura,Elisa Giovannetti,Li Ren

Journal of experimental & clinical cancer research : CR 41:246 PubMed35964097

2022

CD147 mediates epidermal malignant transformation through the RSK2/AP-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Yeye Guo,Ta Xiao,Jie Li,Aiyuan Guo,Li Lei,Chong Jin,Qi Long,Juan Su,Mingzhu Yin,Hong Liu,Chao Chen,Zhe Zhou,Susi Zhu,Juan Tao,Shuo Hu,Xiang Chen,Cong Peng

Cell biology international 45:2086-2095 PubMed34184811

2021

Comparing the frequency of CD33 pSTAT3 myeloid-derived suppressor cells and IL-17 lymphocytes in patients with prostate cancer and benign prostatic hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad-Javad Sanaei,Fatemeh Taheri,Masoud Heshmati,Davood Bashash,Roya Nazmabadi,Faramarz Mohammad-Alibeigi,Mahboobeh Nahid-Samiei,Hedayatollah Shirzad,Nader Bagheri

Cancer medicine 10:5358-5374 PubMed34128342

2021

Immune infiltration phenotypes of prostate adenocarcinoma and their clinical implications.

Applications

Unspecified application

Species

Unspecified reactive species

Zehua Ma,Xiankui Cheng,Ting Yue,Xun Shangguan,Zhixiang Xin,Weiwei Zhang,Jiahua Pan,Qi Wang,Wei Xue

Biology 10: PubMed34200100

2021

Characterization of Tumor-Associated Macrophages and the Immune Microenvironment in Limited-Stage Neuroendocrine-High and -Low Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

David Dora,Christopher Rivard,Hui Yu,Shivaun Lueke Pickard,Viktoria Laszlo,Tunde Harko,Zsolt Megyesfalvi,Elek Dinya,Csongor Gerdan,Gabor Szegvari,Fred R Hirsch,Balazs Dome,Zoltan Lohinai

Frontiers in immunology 10:3104 PubMed32063899

2020

Myeloid-Derived Suppressor Cells and γδT17 Cells Contribute to the Development of Gastric MALT Lymphoma in -Infected Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Zhao,Fei Lu,Jingjing Ye,Min Ji,Yihua Pang,Yan Wang,Lingbo Wang,Guosheng Li,Tao Sun,Jingxin Li,Daoxin Ma,Chunyan Ji

Frontiers in immunology 10:565 PubMed30967878

2019

Short-Term High-Fat Diet Consumption Reduces Hypothalamic Expression of the Nicotinic Acetylcholine Receptor α7 Subunit (α7nAChR) and Affects the Anti-inflammatory Response in a Mouse Model of Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Anelise Cristina Parras Souza,Camilla Mendes Souza,Camila Libardi Amaral,Simone Ferreira Lemes,Leticia Foglia Santucci,Marciane Milanski,Adriana Souza Torsoni,Marcio Alberto Torsoni

The Journal of experimental medicine 216:656-673 PubMed30733283

2019

Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Wenna Jiang,Kaili Zhao,Hongwei Wang,Tianxing Zhou,Weiwei Bai,Xiuchao Wang,Tiansuo Zhao,Chongbiao Huang,Song Gao,Tai Qin,Wenwen Yu,Bo Yang,Xin Li,Danqi Fu,Wei Tan,Shengyu Yang,He Ren,Jihui Hao

Molecular carcinogenesis 57:1651-1663 PubMed30129680

2018

Dual use of hematopoietic and mesenchymal stem cells enhances engraftment and immune cell trafficking in an allogeneic humanized mouse model of head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

John J Morton,Stephen B Keysar,Loni Perrenoud,Tugs-Saikhan Chimed,Julie Reisinger,Brian Jackson,Phuong N Le,Cera Nieto,Karina Gomez,Bettina Miller,Dexiang Gao,Hilary Somerset,Xiao-Jing Wang,Antonio Jimeno

Nature 559:363-369 PubMed29950727

2018

IL-23 secreted by myeloid cells drives castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna Calcinotto,Clarissa Spataro,Elena Zagato,Diletta Di Mitri,Veronica Gil,Mateus Crespo,Gaston De Bernardis,Marco Losa,Michela Mirenda,Emiliano Pasquini,Andrea Rinaldi,Semini Sumanasuriya,Maryou B Lambros,Antje Neeb,Roberta Lucianò,Carlo A Bravi,Daniel Nava-Rodrigues,David Dolling,Tommaso Prayer-Galetti,Ana Ferreira,Alberto Briganti,Antonio Esposito,Simon Barry,Wei Yuan,Adam Sharp,Johann de Bono,Andrea Alimonti

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD33 antibody [6C5/2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (AB11032)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (11)

Product promise