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AB264414

Anti-CD33 antibody [BLR061G]

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Rabbit Recombinant Monoclonal CD33 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CD33 aa 300-400.

대체 명칭 보기

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [BLR061G] (AB264414)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [BLR061G] (AB264414)

Formalin-fixed, paraffin-embedded Acute Myeloid Leukemia tissue stained for CD33 using ab264414 at 1/200 dilution in immunohistochemical analysis. The secondary was an HRP-conjugated goat anti-rabbit IgG antibody. Substrate : DAB.

Immunoprecipitation - Anti-CD33 antibody [BLR061G] (AB264414)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD33 antibody [BLR061G] (AB264414)

CD33 was immunoprecipitated from HEL 92.1.7 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264414 at 20 μl/mg lysate. Western blot was performed on the immunoprecipitate using ab264414 at 1/1000 dilution.

Lane 1 : ab264414 IP in HEL 92.1.7 cell lysate.
Lane 2 : Control IgG IP in HEL 92.1.7 cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-CD33 antibody [BLR061G] (ab264414)

Predicted band size: 40 kDa

true

Exposure time: 10s

Western blot - Anti-CD33 antibody [BLR061G] (AB264414)
  • WB

Supplier Data

Western blot - Anti-CD33 antibody [BLR061G] (AB264414)

All lanes:

Western blot - Anti-CD33 antibody [BLR061G] (ab264414) at 1/1000 dilution

Lane 1:

HEL 92.1.7 whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 3:

My-La CD4+ whole cell lysate at 50 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 6:

THP-1 (human monocytic leukemia cell line) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 40 kDa

true

Exposure time: 30s

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR061G

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CD33 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P20138

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대체품

Primary Antibodies

AB269306

Alexa Fluor® 700 Anti-CD33 antibody [WM53]

Flow Cytometry - Alexa Fluor® 700 Anti-CD33 antibody [WM53] (AB269306)

  • 0
  • 0
Primary Antibodies

AB134115

Anti-CD33 antibody [EPR4423]

20ul selling size|RabMAb|KO Validated|Recombinant

Flow Cytometry - Anti-CD33 antibody [EPR4423] (AB134115)

  • 0
  • 0
Primary Antibodies

AB264558

Anti-CD33 antibody [EPR23052-5]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD33 antibody [EPR23052-5] (AB264558)

  • 0
  • 0
Primary Antibodies

AB264572

Anti-CD33 antibody [EPR23052-5] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (AB264572)

  • 0
  • 0
Primary Antibodies

AB215383

Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free

KO Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-CD33 antibody [EPR4423] - Low endotoxin, Azide free (AB215383)

  • 0
  • 0
Primary Antibodies

AB214439

Anti-CD33 antibody [EPR4423] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD33 antibody [EPR4423] - BSA and Azide free (AB214439)

  • 5
  • 1
Primary Antibodies

AB252263

Anti-CD33 antibody [WM53] - BSA and Azide free

Recombinant

Flow Cytometry - Anti-CD33 antibody [WM53] - BSA and Azide free (AB252263)

  • 0
  • 0

보완 제품

Assay Kits

AB283542

Human CD33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD33 ELISA Kit (AB283542)

  • 0
  • 0
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB227019

Anti-CD19 antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody (AB227019)

  • 0
  • 1
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20 μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE sections.</p>" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).
See full target information CD33
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of biological sciences 17:3320-3330 PubMed34512149

2021

NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis inhibiting the CTHRC1/HIF-1α axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Yang Yang,Haiying Yan,Xiaying Peng,Jun Zou
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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