AB245698

Anti-CD33 antibody [hP67.6 (Gemtuzumab)]

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Rabbit Monoclonal CD33 antibody. Suitable for Flow Cyt, ELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD33 protein.

대체 명칭 보기

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

2 이미지

Flow Cytometry - Anti-CD33 antibody [hP67.6 (Gemtuzumab)] (AB245698)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD33 antibody [hP67.6 (Gemtuzumab)] (AB245698)

Human leukocytes were stained with an isotype control (Panel A) or ab245698 (Panel B) at a concentration of 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

ELISA - Anti-CD33 antibody [hP67.6 (Gemtuzumab)] (AB245698)

ELISA

Supplier Data

ELISA - Anti-CD33 antibody [hP67.6 (Gemtuzumab)] (AB245698)

ELISA Plate coated with human His-tagged CD33 at a concentration of 2 μg/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using ab245698. For detection, a 1/4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.

This data was generated using the original Human IgG1 kappa version of this clone.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

hP67.6 (Gemtuzumab)

Isotype

IgG

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, ELISA

applications

Immunogen

Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information.

P20138

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.0001-10 µg/mL", "ELISA-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.02% Proclin 300 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).

See full target information CD33

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Neural regeneration research 20:491-502 PubMed38819062

2024

Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Feng,Juanji Li,Tingting Liu,Shiqi Huang,Xiangliang Chen,Shen Liu,Junshan Zhou,Hongdong Zhao,Ye Hong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD33 antibody [hP67.6 (Gemtuzumab)]

Flow Cytometry - Anti-CD33 antibody [hP67.6 (Gemtuzumab)] (AB245698)

ELISA - Anti-CD33 antibody [hP67.6 (Gemtuzumab)] (AB245698)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise