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AB240961

Anti-CD35 antibody [SP197] - BSA and Azide free

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(1 출판물)

Rabbit Recombinant Monoclonal CD35 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CD35, C3BR, CR1, Complement receptor type 1, C3b/C4b receptor

6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD35 with ab183359 at 1/100 dilution (1.77 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the glomerular podocytes in the human kidney, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183359 for 10 mins at room temperature.

This image was generated using ab183359, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD35 with ab183359 at 1/100 dilution (1.77 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183359 for 10 mins at room temperature.

This image was generated using ab183359, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue sections labeling CD35 using ab183359 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue sections labeling CD35 using ab183359 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue sections labeling CD35 using ab183359 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP197] - BSA and Azide free (AB240961)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon tissue sections labeling CD35 using ab183359 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183359)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP197

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB212311

Anti-CD35 antibody [CR1/802] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [CR1/802] - BSA and Azide free (AB212311)

  • 0
  • 0
Primary Antibodies

AB178451

PE Anti-CD35 antibody [E11]

Flow Cytometry - PE Anti-CD35 antibody [E11] (AB178451)

  • 0
  • 0
Primary Antibodies

AB25

Anti-CD35 antibody [E11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [E11] (AB25)

  • 3
  • 1
Primary Antibodies

AB245683

Anti-CD35 antibody [8C12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [8C12] (AB245683)

  • 0
  • 0
Primary Antibodies

AB229875

Anti-CD35 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody (AB229875)

  • 0
  • 0
Primary Antibodies

AB227666

Anti-CD35 antibody [SP191]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)

  • 0
  • 0
Primary Antibodies

AB126737

Anti-CD35 antibody [EPR6602]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] (AB126737)

  • 0
  • 0
Primary Antibodies

AB133293

Anti-CD35 antibody [EPR6601]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6601] (AB133293)

  • 0
  • 0
Primary Antibodies

AB236236

Anti-CD35 antibody [SP191] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] - BSA and Azide free (AB236236)

  • 0
  • 0
Primary Antibodies

AB269303

Alexa Fluor® 488 Anti-CD35 antibody [E11]

Flow Cytometry - Alexa Fluor® 488 Anti-CD35 antibody [E11] (AB269303)

  • 0
  • 0
Primary Antibodies

AB243085

APC Anti-CD35 antibody [E11]

Flow Cytometry - APC Anti-CD35 antibody [E11] (AB243085)

  • 0
  • 0

보완 제품

Assay Kits

AB277439

Human CD35 ELISA Kit

ELISA - Human CD35 ELISA Kit (AB277439)

  • 0
  • 0
Primary Antibodies

AB109493

Anti-IgG4 antibody [EP4420]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (AB109493)

  • 0
  • 0
Primary Antibodies

AB228461

Anti-BRAF (mutated V600E) antibody [VE1]

20ul selling size|BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [VE1] (AB228461)

  • 4
  • 7
Primary Antibodies

AB108307

Anti-CD46 antibody [EPR4014]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

  • 5
  • 1
Primary Antibodies

AB133684

Anti-CD55 antibody [EPR6689]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (AB133684)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab240961 is the carrier-free version of ab183359.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS by protein A/G.
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed : 2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed : 2963069). Also acts in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed : 2972794, PubMed : 8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed : 25742728).. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
See full target information CR1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 21:2209-2219 PubMed32186759

2020

PDGFD induces ibrutinib resistance of diffuse large B‑cell lymphoma through activation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Jin,Leiping Wang,Zhonghua Tao,Jian Zhang,Fangfang Lv,Junning Cao,Xichun Hu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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