Anti-CD37 antibody [EPR25397-149]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD37 with ab300400 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD37 with ab300400 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control a. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Cells were stained with rabbit IgG or ab300400. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD37 with ab300400 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control : MCF7 (PMID : 33652767).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymp tissue labeling CD37 with ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining was found in human Hodgkin's lymphoma. The section was incubated with ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling CD37 with ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining was found on human skeletal muscle. The section was incubated with ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD37 with ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining was found in human tonsil (PMID : 25934707). The section was incubated with ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (AB300400)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD37 with ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining was found in human spleen (PMID : 25934707). The section was incubated with ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR25397-149] (AB300400)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD37 with ab300400 at 1/500 (1.086 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. Negative control : MCF7 (PMID : 33652767). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IP

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Immunoprecipitation - Anti-CD37 antibody [EPR25397-149] (AB300400)

CD37 was immunoprecipitated from 0.35 mg Human tonsil tissue lysate 10 ug with ab300400 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300400 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human tonsil tissue lysate 10 ug

Lane 2 : ab300400 IP in Human tonsil tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300400 in Human tonsil tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-CD37 antibody [EPR25397-149] (ab300400) at 1/1000 dilution

All lanes:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 15s

• WB

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Western blot - Anti-CD37 antibody [EPR25397-149] (AB300400)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD37 antibody [EPR25397-149] (ab300400) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate

Lane 2:

Human skeletal muscle tissue lysate

Lane 3:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa

false