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AB183326

Anti-CD38 antibody [SP149] - C-terminal

3

(3 리뷰들)

|

(4 제품이 사용된 논문 )

Anti-CD38 antibody [SP149] - C-terminal (ab183326) is a rabbit monoclonal antibody detecting CD38 in Flow Cytometry, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1

17 이미지
Flow Cytometry - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Flow cytometric analysis of rabbit anti-CD38 C terminal (SP149) ab183326 (1/100) antibody in HeLa cells (green) compared to negative control of rabbit IgG (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CD38 with ab183326 at 1/100 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183326 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD38 with ab183326 at 1/100 dilution. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on sporadic cells in human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183326 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human HK Lymphoma tissue labeling CD38 with ab183326 at 1/100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human Stomach Adenocarcinoma tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human ovary tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human Colon Adenocarcinoma tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD38 with ab183326 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil labelling CD38 with ab236232 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab236232 anti C38 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation (ab236232).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD38 with ab183326 at 1/100.

관련 conjugated 항체와 다양한 조성의 항체 (11)

  • Carrier free

    Anti-CD38 antibody [SP149] - BSA and Azide free

  • 660 APC

    APC Anti-CD38 antibody [SP149] (C-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD38 antibody [SP149] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD38 antibody [SP149] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD38 antibody [SP149] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD38 antibody [SP149] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD38 antibody [SP149] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CD38 antibody [SP149] - C-terminal

  • Alkaline Phosphatase

    Alkaline Phosphatase Anti-CD38 antibody [SP149] - C-terminal

  • HRP

    HRP Anti-CD38 antibody [SP149] (C-terminal)

  • 578 PE

    PE Anti-CD38 antibody [SP149] (C-terminal)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP149

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

제품 세부 정보

What is this antibody validated in?
Anti-CD38 antibody [SP149] - C-terminal (ab183326) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Collaboration
Anti-CD38 [SP149] - C-terminal (ab183326) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [SP149] also available for your convenience: ab183326, Carrier free - ab236232, ab279486, PE - ab306156, APC - ab306157, HRP - ab306158, Alkaline Phosphatase - ab308991, Alexa Fluor® 488 - ab309934, Alexa Fluor® 647 - ab310301, Alexa Fluor® 594 - ab310764, Alexa Fluor® 555 - ab312291, Alexa Fluor® 568 - ab312786, Alexa Fluor® 750 - ab321269

특성 및 보관 정보

제형
Liquid
Purity
Tissue culture supernatant
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS by protein A/G.
보관 버퍼
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Multifunctional transmembrane glycoprotein able to exert enzymatic activities and also to mobilize calcium, to transduce signals, to adhere to hyaluronan and to other ligands. Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed : 7961800, PubMed : 8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed : 16690024). Has cADPR hydrolase activity (PubMed : 7961800, PubMed : 8253715). Functions also as a receptor that binds the ligand CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and migration across the endothelial barrier (PubMed : 9551996). Involved in the regulation of crucial dendritic cell functions acquired at the mature stage, such as CCL21-driven migration, survival, and Th1-polarizing activity (PubMed : 16293598). In lamina propria T lymphocytes, CD38/CD31 cognate interactions initiate a multistep signaling pathway resulting in activation of LCK anf LAT, followed by cytokine release (PubMed : 11259373).
See full target information CD38

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:5423 PubMed38926338

2024

MRE11 and TREX1 control senescence by coordinating replication stress and interferon signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hervé Técher,Diyavarshini Gopaul,Jonathan Heuzé,Nail Bouzalmad,Baptiste Leray,Audrey Vernet,Clément Mettling,Jérôme Moreaux,Philippe Pasero,Yea-Lih Lin

HemaSphere 8:e32 PubMed38434534

2024

CDK6 protein expression is associated with disease progression and treatment resistance in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Steinhart,Peter Möller,Miriam Kull,Jan Krönke,Thomas F E Barth

European journal of nuclear medicine and molecular imaging 48:2749-2760 PubMed33543326

2021

ImmunoPET imaging of multiple myeloma with [Ga]Ga-NOTA-Nb1053.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Yumei Chen,Yun Nan Hou,Qiufang Liu,Di Zhang,Haitao Zhao,You Zhang,Shuxian An,Lianghua Li,Jian Hou,Gang Huang,Jianjun Liu,Yong Juan Zhao,Weijun Wei

AIDS (London, England) 33:2125-2136 PubMed31335802

2019

Pre-exposure prophylaxis differentially alters circulating and mucosal immune cell activation in herpes simplex virus type 2 seropositive women.

Applications

Unspecified application

Species

Unspecified reactive species

Laura E Richert-Spuhler,Laura Pattacini,Margot Plews,Elizabeth Irungu,Timothy R Muwonge,Elly Katabira,Nelly Mugo,Adrienne F A Meyers,Connie Celum,Jared M Baeten,Jairam R Lingappa,Jennifer M Lund
제품이 사용된 논문 모두 보기

Product promise

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