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AB243872

Anti-CD4 antibody [BL-155-1C11] - BSA free

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Rabbit Recombinant Monoclonal CD4 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD4.

대체 명칭 보기

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [BL-155-1C11] - BSA free (AB243872)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [BL-155-1C11] - BSA free (AB243872)

Formalin-fixed, paraffin-embedded human appendix tissue stained for CD4 using ab243872 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

Immunoprecipitation - Anti-CD4 antibody [BL-155-1C11] - BSA free (AB243872)
  • IP

Unknown

Immunoprecipitation - Anti-CD4 antibody [BL-155-1C11] - BSA free (AB243872)

CD4 was immunoprecipitated from 1 mg MOLT-4 whole cell lysate with ab243872 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243872 at 1/1000 dilution. Lane 1 : ab243872 IP in MOLT-4 cell lysate.

Lane 2 : Contol IgG in MOLT-4 cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

Predicted band size: 51 kDa

false

Western blot - Anti-CD4 antibody [BL-155-1C11] - BSA free (AB243872)
  • WB

Unknown

Western blot - Anti-CD4 antibody [BL-155-1C11] - BSA free (AB243872)

Western blot analysis using ab243872 at 1/1000 dilution.

Lane 1 : Jurkat whole cell lysate (15 µg).

Lane 2 : MOLT-4 whole cell lysate (15 µg).

Lane3 : U-937 whole cell lysate (15 µg).

Lane 4 : Ramos whole cell lysate (15 µg).

Lane 5 : SR whole cell lysate (15 µg).

Lane 6 : HL-60 whole cell lysate (15 µg).

Lane 7 : HeLa whole cell lysate (15µg).

Lane 8 : HEK-293T whole cell lysate (15 µg).

Lane 9 : HEPG2 whole cell lysate (15 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 30 seconds

All lanes:

Western blot - Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

Predicted band size: 51 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-155-1C11

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CD4. The exact immunogen used to generate this antibody is proprietary information.

P01730

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대체품

Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

  • 4
  • 2
Primary Antibodies

AB181724

Anti-CD4 antibody [EPR6855] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] - BSA and Azide free (AB181724)

  • 4
  • 9
Primary Antibodies

AB238798

Anti-CD4 antibody [SP35] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-CD4 antibody [SP35] - BSA and Azide free (AB238798)

  • 0
  • 0
Primary Antibodies

AB221775

Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB277931

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (AB277931)

  • 0
  • 0

보완 제품

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Primary Antibodies

AB282458

Anti-PD-L1 antibody [RM1012]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [RM1012] (AB282458)

  • 0
  • 0
Primary Antibodies

AB137132

Anti-PD1 antibody [EPR4877(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] (AB137132)

  • 5
  • 5
Primary Antibodies

AB209205

Anti-Granzyme A antibody [EPR20161]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme A antibody [EPR20161] (AB209205)

  • 0
  • 0
Primary Antibodies

AB237712

Anti-CTLA4 antibody [CAL49]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] (AB237712)

  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 14:5147 PubMed37620345

2023

RNAPII-dependent ATM signaling at collisions with replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Einig,Chao Jin,Valentina Andrioletti,Boris Macek,Nikita Popov
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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