Anti-CD42b antibody [AK2] - BSA and Azide free

• Flow Cyt

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Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD42b with ab61402 at 1/490 (Right) compared with a Mouse IgG isotype control (Left). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab61402 (Right). Then stained with anti-CD41 conjugated to APC. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEL (human Erythroleukemia erythroblast) cells labelling CD42b with ab61402 at 1/50 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HEL cells. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

The negative controls are as follows :
-ve control 1 : ab61402 at 1/100 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes cells labelling CD42b with ab61402 at 1/50 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in mouse splenocytes. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

The negative controls are as follows :
-ve control 1 : ab61402 at 1/100 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1 : 1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab61402).