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AB244554

Anti-CD45 antibody [OX30] - BSA and Azide free

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Mouse Monoclonal CD45 antibody. Carrier free. Suitable for Flow Cyt and reacts with Rat samples. Cited in 1 publication.

대체 명칭 보기

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 이미지
Flow Cytometry - Anti-CD45 antibody [OX30] - BSA and Azide free (AB244554)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD45 antibody [OX30] - BSA and Azide free (AB244554)

This data was generating using the same clone in a different formulation (ab6329).

Lewis rat splenocytes stained with ab6329 (right) or mouse IgG2aκ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6329) or mouse IgG2aκ Isotype (ab18450) (1x106 in 100μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OX30

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB40763

Anti-CD45 antibody [EP322Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] (AB40763)

  • 5
  • 15
Primary Antibodies

AB208022

Anti-CD45 antibody [EPR20033]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] (AB208022)

  • 4
  • 5
Primary Antibodies

AB200317

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

  • 0
  • 1
Primary Antibodies

AB214437

Anti-CD45 antibody [EP322Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EP322Y] - BSA and Azide free (AB214437)

  • 5
  • 1

보완 제품

Assay Kits

AB277445

Mouse CD45 ELISA kit

ELISA - Mouse CD45 ELISA kit (AB277445)

  • 0
  • 0
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB81289

Anti-CD34 antibody [EP373Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (AB81289)

  • 4
  • 56
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

  • 5
  • 17
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244554 is the carrier-free version of ab6329.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from TCS.
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Environmental toxicology 36:1654-1663 PubMed33969609

2021

Lysosome Fe release is responsible for etoposide- and cisplatin-induced stemness of small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kangwu Wang,Xiao Chen,Wang Zuyi,Liucheng Chen,Wei Fu
제품이 사용된 논문 모두 보기
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Product promise

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