AB193378

Anti-CD5 antibody [CD5/54/F6]

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(2 제품이 사용된 논문 )

Mouse Monoclonal CD5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD5.

대체 명칭 보기

CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] (AB193378)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] (AB193378)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human tonsil tissue labeling CD5 with ab193378 at 1 μg/ml.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CD5/54/F6

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CD5. The exact immunogen used to generate this antibody is proprietary information.

P06127

Specificity

ab193378 is very useful in differentiating among mature small lymphoid cell malignancies. It can also be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.

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대체품

Primary Antibodies

AB213003

Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free (AB213003)

Primary Antibodies

AB213004

Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free (AB213004)

Primary Antibodies

AB200697

Anti-CD5 antibody [C5/473 + CD5/54/F6]

Primary Antibodies

AB269293

PE Anti-CD5 antibody [L17F12]

Flow Cytometry - PE Anti-CD5 antibody [L17F12] (AB269293)

Primary Antibodies

AB269292

PE Anti-CD5 antibody [CRIS1]

Flow Cytometry - PE Anti-CD5 antibody [CRIS1] (AB269292)

Primary Antibodies

AB16699

Anti-CD5 antibody [SP19]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] (AB16699)

Primary Antibodies

AB134174

Anti-CD5 antibody [EPR2953]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CD5 antibody [EPR2953] (AB134174)

Primary Antibodies

AB75877

Anti-CD5 antibody [EP2952]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (AB75877)

Primary Antibodies

AB194821

Anti-CD5 antibody [EP2952] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (AB194821)

Primary Antibodies

AB239796

Anti-CD5 antibody [SP19] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] - BSA and Azide free (AB239796)

Primary Antibodies

AB155375

PE/Cy7® Anti-CD5 antibody [UCHT2]

Flow Cytometry - PE/Cy7® Anti-CD5 antibody [UCHT2] (AB155375)

보완 제품

Assay Kits

AB314852

Human CD5 ELISA Kit - Intracellular

SimpleStep

Sandwich ELISA - Human CD5 ELISA Kit - Intracellular (AB314852)

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB16702

Anti-CD23 antibody [SP23]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

Primary Antibodies

AB183326

Anti-CD38 antibody [SP149] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD38 antibody [SP149] - C-terminal (AB183326)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.

Biological function summary

CD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.

Pathways

CD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.

CD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed : 1384049, PubMed : 1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed : 23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed : 27499044).

See full target information CD5

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Circulation. Heart failure 16:e010294 PubMed37465947

2023

Inhibition of Pyk2 Improves Cx43 Intercalated Disc Localization, Infarct Size, and Cardiac Function in Rats With Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zheng,Gaelle Spagnol,Devashri R Gandhi,Kanika Sharma,Vikas Kumar,Kaushik P Patel,Paul L Sorgen

Cancers 13: PubMed34638404

2021

Towards a Better Characterisation of Leukemic Cells in Chronic Lymphocytic Leukaemia: Cell-Size Heterogeneity Reflects Their Activation Status and Migratory Abilities.

Applications

Unspecified application

Species

Unspecified reactive species

Gayane Manukyan,Zuzana Mikulkova,Peter Turcsanyi,Jakub Savara,Markéta Trajerová,Zuzana Kubova,Tomas Papajik,Eva Kriegova

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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