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AB248712

Anti-CD5 antibody [EPR2953] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD5 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1

1 이미지
Western blot - Anti-CD5 antibody [EPR2953] - BSA and Azide free (AB248712)
  • WB

Unknown

Western blot - Anti-CD5 antibody [EPR2953] - BSA and Azide free (AB248712)

This data was developed using ab134174, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD5 antibody [EPR2953] (<a href='/ko/products/primary-antibodies/cd5-antibody-epr2953-ab134174'>ab134174</a>) at 1/1000 dilution

All lanes:

TPA treated PBMC (Human peripheral blood mononuclear cell), whole cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2953

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB213003

Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free (AB213003)

  • 0
  • 0
Primary Antibodies

AB213004

Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free (AB213004)

  • 0
  • 0
Primary Antibodies

AB269293

PE Anti-CD5 antibody [L17F12]

Flow Cytometry - PE Anti-CD5 antibody [L17F12] (AB269293)

  • 0
  • 0
Primary Antibodies

AB269292

PE Anti-CD5 antibody [CRIS1]

Flow Cytometry - PE Anti-CD5 antibody [CRIS1] (AB269292)

  • 0
  • 0
Primary Antibodies

AB238467

Anti-CD5 antibody [OX19]

Trial Size

Flow Cytometry - Anti-CD5 antibody [OX19] (AB238467)

  • 0
  • 0
Primary Antibodies

AB244582

Anti-CD5 antibody [OX19] - BSA and Azide free

Flow Cytometry - Anti-CD5 antibody [OX19] - BSA and Azide free (AB244582)

  • 0
  • 0

보완 제품

Assay Kits

AB309429

Human CD5 ELISA Kit

Sandwich ELISA - Human CD5 ELISA Kit (AB309429)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB109296

Anti-CD7 antibody [EPR4242]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (AB109296)

  • 0
  • 0
Primary Antibodies

AB101533

Anti-CD43 antibody [SP55]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (AB101533)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab248712 is the carrier-free version of ab134174.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.
Biological function summary

CD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.

Pathways

CD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.

CD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed : 1384049, PubMed : 1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed : 23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed : 27499044).
See full target information CD5
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 14:4467 PubMed37491377

2023

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhong,Xujia Wu,Yixin Gao,Junju Chen,Maolei Zhang,Huangkai Zhou,Jia Yang,Feizhe Xiao,Xuesong Yang,Nunu Huang,Haoyue Qi,Xiuxing Wang,Fan Bai,Yu Shi,Nu Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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