AB82749

Anti-CD57 antibody [NK1], prediluted

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(4 제품이 사용된 논문 )

Mouse Monoclonal CD57 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD57 protein.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [NK1], prediluted (AB82749)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [NK1], prediluted (AB82749)

Paraffin embedded human tonsil tissue stained for CD57 using ab82749 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CD57 antibody [NK1], prediluted (AB82749)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD57 antibody [NK1], prediluted (AB82749)

ICC/IF image of ab82749 stained U2OS cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82749 at Undiluted (Used Neat) overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- mouse (ab98674) IgM preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

NK1

Isotype

IgM

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to a 110kD human CD57 myeloid associated glycoprotein. It stains mononuclear cells with natural killer activity and neuroendocrine cells and their tumors.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1", "ICCIF-species-notes": "<p>Use Neat</p>" } } }

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD57 also known as HNK-1 is a glycoprotein with a molecular weight of approximately 110 to 150 kDa. It is a marker expressed on the surface of natural killer (NK) cells T lymphocytes and some subsets of CD8+ T cells. Besides you can find this marker in neural tissue. As a surface marker CD57 is used in human immunohistochemistry (IHC) studies to identify cell populations in immune responses and has significant utility in the characterization of lymphocyte subsets.

Biological function summary

CD57 plays a role in immune regulation and cell-mediated cytotoxicity. CD57+ cells which are often mature or post-activation states in NK cells and T lymphocytes are less proliferative but have enhanced cytotoxic abilities. These cells contribute to the immune surveillance against viral infections and tumor cells. CD57 does not belong to any well-known protein complexes but can cooperate with other surface markers to modulate immune functions.

Pathways

CD57+ cell populations participate in the immune system pathways related to cell-mediated immunity and cytotoxic response. The presence of CD57 is often associated with the downregulation of proliferative pathways and the enhancement of cytotoxic pathways. It shows functional overlap with proteins such as CD16 and NKG2D which also play roles in mediating the cytotoxic activity of NK cells.

CD57 is connected to chronic infections and certain autoimmune conditions. In chronic viral infections like HIV CD57+ T cells show a state of replicative senescence where their persistent expression links to disease progression. In autoimmune disorders like rheumatoid arthritis CD57+ T cells are found in the inflamed tissues. This protein works alongside CD8 in conditions of immune dysfunction providing insights into immune status and disease activity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information CD57

대체 명칭 보기

HKN1, LEU7

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Therapeutic advances in medical oncology 16:17588359241297080 PubMed39563715

2024

The AFCRPLITY score for predicting the prognosis of immunotherapy combined with local-regional therapy in unresectable hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxuan Zuo,Ran Wei,Da Li,Wang Li,Chao An

BMC immunology 25:51 PubMed39068393

2024

Histone acetylation risk model predicts prognosis and guides therapy selection in glioblastoma: implications for chemotherapy and anti-CTLA-4 immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyi Jin,Zhigang Qin,Hang Zhao

Frontiers in oncology 14:1288820 PubMed38841168

2024

Deciphering glycosylation-driven prognostic insights and therapeutic prospects in glioblastoma through a comprehensive regulatory model.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyi Jin,Zhuo Chen,Hang Zhao

BMC cancer 17:375 PubMed28549420

2017

Prognostic significance of tumor infiltrating immune cells in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Fang,Xiaoxu Li,Da Ma,Xiangqi Liu,Yichen Chen,Yun Wang,Vivian Wai Yan Lui,Juan Xia,Bin Cheng,Zhi Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD57 antibody [NK1], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [NK1], prediluted (AB82749)

Immunocytochemistry/ Immunofluorescence - Anti-CD57 antibody [NK1], prediluted (AB82749)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise