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AB267422

Anti-CD59 antibody [EPR22394-40] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CD59 antibody. Carrier free. Suitable for sELISA and reacts with Human, Rat samples.

대체 명칭 보기

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

1 이미지
Sandwich ELISA - Anti-CD59 antibody [EPR22394-40] - BSA and Azide free (Detector) (AB267422)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD59 antibody [EPR22394-40] - BSA and Azide free (Detector) (AB267422)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab263893).

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Biotin

    Biotin Anti-CD59 antibody [EPR22394-40] (Detector)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22394-40

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB263893

Human CD59 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD59 ELISA Kit (AB263893)

  • 0
  • 0
Primary Antibodies

AB259551

Anti-LRG1/LRG antibody [EPR22432-122] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-LRG1/LRG antibody [EPR22432-122] - BSA and Azide free (Capture) (AB259551)

  • 0
  • 0
Primary Antibodies

AB244958

Anti-Bag3 antibody [EPR20207-109] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Bag3 antibody [EPR20207-109] - BSA and Azide free (Detector) (AB244958)

  • 0
  • 0
Primary Antibodies

AB252631

Anti-Serum Amyloid P/SAP antibody [EPR20042-239] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Serum Amyloid P/SAP antibody [EPR20042-239] - BSA and Azide free (Detector) (AB252631)

  • 0
  • 0
Primary Antibodies

AB245087

Anti-Vaspin antibody [EPR21830-62] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Vaspin antibody [EPR21830-62] - BSA and Azide free (Detector) (AB245087)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab267422 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD59. The recommended pair for sandwich ELISA is:
Capture: ab267421, Human CD59 Capture Antibody (unconjugated)
Detector: ab267422, Human CD59 Detector Antibody (unconjugated)
The reference range value is 23.4375 - 1500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.
See full target information CD59
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Product promise

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