JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB253240

Anti-CD62L antibody [EPR17012-27]

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal CD62L antibody. Suitable for Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

대체 명칭 보기

CD62L, Lnhr, Ly-22, Ly22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1

2 이미지
Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (AB253240)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (AB253240)

Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

Gated on viable CD4+ T cells.

Western blot - Anti-CD62L antibody [EPR17012-27] (AB253240)
  • WB

Unknown

Western blot - Anti-CD62L antibody [EPR17012-27] (AB253240)

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 12165530).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 75-100 kDa

false

Exposure time: 3min

관련 conjugated 항체와 다양한 조성의 항체 (4)

  • Carrier free

    Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (Capture)

  • Carrier free

    Anti-CD62L antibody [EPR17012-27] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD62L antibody [EPR17012-27]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD62L antibody [EPR17012-27]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17012-27

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB238473

Anti-CD62L antibody [OX85]

Trial Size

Flow Cytometry - Anti-CD62L antibody [OX85] (AB238473)

  • 0
  • 0
Primary Antibodies

AB272170

Anti-CD62L antibody [EPR23565-109] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] - BSA and Azide free (AB272170)

  • 0
  • 0
Primary Antibodies

AB245239

Anti-CD62L antibody [EPR23565-109]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)

  • 0
  • 0
Primary Antibodies

AB269290

Alexa Fluor® 700 Anti-CD62L antibody [DREG-56]

Flow Cytometry - Alexa Fluor® 700 Anti-CD62L antibody [DREG-56] (AB269290)

  • 0
  • 0
Primary Antibodies

AB244587

Anti-CD62L antibody [OX85] - BSA and Azide free

Flow Cytometry - Anti-CD62L antibody [OX85] - BSA and Azide free (AB244587)

  • 0
  • 0

보완 제품

Assay Kits

AB199085

Mouse L-Selectin ELISA Kit (CD62L)

Recombinant|SimpleStep

Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (AB199085)

  • 0
  • 0
Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

  • 4
  • 1
Primary Antibodies

AB316113

Anti-PSGL-1 antibody [EPR28170-27]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (AB316113)

  • 0
  • 0
Primary Antibodies

AB319105

Anti-CD62E antibody [EPR28605-81]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody [EPR28605-81] (AB319105)

  • 0
  • 0
Primary Antibodies

AB222736

Anti-ICAM1 antibody [EPR22161-284]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR22161-284] (AB222736)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/60", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab253240 (EPR17012-27) is the capture of the ssELISA kit ab199085.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed : 1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).
See full target information Sell
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PeerJ 13:e19215 PubMed40183048

2025

Temporal and spatial distribution of histone acetylation in mouse molar development.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Du,Wanyi Luo,Liwei Zheng,Xuedong Zhou,Wei Du
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com