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AB245239

Anti-CD62L antibody [EPR23565-109]

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Rabbit Recombinant Monoclonal CD62L antibody. Suitable for Flow Cyt and reacts with Human samples.

대체 명칭 보기

CD62L, LNHR, LYAM1, SELL, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, TQ1, gp90-MEL, LAM-1, LECAM1

3 이미지
Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)

Flow cytometric analysis of Human peripheral blood mononuclear (PBMC) cells labelling CD62L with ab245239 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as secondary antibody at 1/2000 dilution.

Cells were stained with rabbit IgG (Left) or ab245239 (Right). Then stained with anti-CD4 conjugated to BV421 and anti-CD45RO (Alexa Fluor®647). Gated on viable CD4+ T cells.

Characterization of T cell phenotype was consistent with literature. (PMID : 27562135).

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)

Flow cytometric analysis of Human peripheral blood mononuclear (PBMC) cells labelling CD62L with ab245239 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab245239 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Gated on viable lymphocytes.

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD62L antibody [EPR23565-109] (AB245239)

Flow cytometric analysis of Human peripheral blood mononuclear (PBMC) cells labelling CD62L with ab245239 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Carrier free

    Anti-CD62L antibody [EPR23565-109] - BSA and Azide free

  • 660 APC

    APC Anti-CD62L antibody [EPR23565-109]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD62L antibody [EPR23565-109]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23565-109

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB253240

Anti-CD62L antibody [EPR17012-27]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (AB253240)

  • 0
  • 0
Primary Antibodies

AB253241

Anti-CD62L antibody [EPR17012-35]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (AB253241)

  • 0
  • 0
Primary Antibodies

AB254204

Anti-CD62L antibody [EPR17012-35] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (AB254204)

  • 0
  • 0
Primary Antibodies

AB254203

Anti-CD62L antibody [EPR17012-27] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (AB254203)

  • 0
  • 0

보완 제품

Assay Kits

AB314373

Human CD62L ELISA Kit

SimpleStep

Sandwich ELISA - Human CD62L ELISA Kit (AB314373)

  • 0
  • 0
Primary Antibodies

AB253187

Anti-CCR7 antibody [EPR23192-57]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

  • 0
  • 0
Primary Antibodies

AB183354

Anti-CD16 antibody [SP175]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [SP175] (AB183354)

  • 5
  • 2
Primary Antibodies

AB131254

Anti-CD27 antibody [EPR8569]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8569] (AB131254)

  • 5
  • 2
Primary Antibodies

AB281586

Anti-CD45 antibody [RM1007]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed : 12403782, PubMed : 28011641, PubMed : 28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed : 12403782, PubMed : 28011641).
See full target information SELL
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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