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AB244587

Anti-CD62L antibody [OX85] - BSA and Azide free

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Mouse Monoclonal CD62L antibody. Carrier free. Suitable for Flow Cyt and reacts with Rat samples. Cited in 1 publication.

대체 명칭 보기

CD62L, Lnhr, Ly-22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1

1 이미지
Flow Cytometry - Anti-CD62L antibody [OX85] - BSA and Azide free (AB244587)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD62L antibody [OX85] - BSA and Azide free (AB244587)

This data was generating using the same clone in a different formulation (ab238473)

Lewis rat splenocytes stained with ab238473 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238473) or mouse IgG1κ Isotype (ab170190) (1x106 in 100μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OX85

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB253240

Anti-CD62L antibody [EPR17012-27]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (AB253240)

  • 0
  • 0
Primary Antibodies

AB253241

Anti-CD62L antibody [EPR17012-35]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (AB253241)

  • 0
  • 0
Primary Antibodies

AB254204

Anti-CD62L antibody [EPR17012-35] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (AB254204)

  • 0
  • 0
Primary Antibodies

AB254203

Anti-CD62L antibody [EPR17012-27] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (AB254203)

  • 0
  • 0

보완 제품

Assay Kits

AB45917

Human CD62L ELISA Kit (Selectin L)

Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)

  • 0
  • 0
Primary Antibodies

AB316113

Anti-PSGL-1 antibody [EPR28170-27]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (AB316113)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB222736

Anti-ICAM1 antibody [EPR22161-284]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR22161-284] (AB222736)

  • 0
  • 0
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244587 is the carrier-free version of ab238473.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from TCS.
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
See full target information Sell
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of the National Cancer Institute 65:33-42 PubMed6993744

1980

Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

K F Bradstock,G Janossy,G Pizzolo,A V Hoffbrand,A McMichael,J R Pilch,C Milstein,P Beverley,F J Bollum
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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