AB259996

Anti-CD62P antibody [EPR22850-206] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal CD62P antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CD62P, GMRP, GRMP, SELP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM

1 이미지

Flow Cytometry - Anti-CD62P antibody [EPR22850-206] - BSA and Azide free (AB259996)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD62P antibody [EPR22850-206] - BSA and Azide free (AB259996)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / HEL (human Erythroleukemia erythroblast, Right) cells labelling CD62P with ab255821 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : HEK-293 (PMID : 7693674).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255821).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22850-206

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab259996 is the carrier-free version of ab255821.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD62P also known as P-selectin is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It is a type of protein with a known molecular weight of approximately 140 kDa. P-selectin plays a mechanical role in mediating leukocyte rolling on the endothelium which is an initial step in the inflammatory response. This protein is rapidly translocated to the plasma membrane upon platelet activation and is stored in alpha granules of platelets and Weibel-Palade bodies of endothelial cells.

Biological function summary

P-selectin is critical in the recruitment of leukocytes to sites of tissue injury and infection. It facilitates the adhesion of leukocytes to the vascular endothelium by binding to its ligand PSGL-1 on the leukocyte surface. This interaction supports the transition of leukocytes from the circulation to the site of inflammation. P-selectin is also involved in thrombus formation as it contributes to the interactions between platelets leukocytes and endothelium in the vascular wall. It acts as part of a dynamic complex in the regulation of the innate immune response and hemostasis.

Pathways

CD62P plays an important role in both the inflammatory and coagulation pathways. It is an important mediator in the leukocyte adhesion cascade influencing leukocyte tethering and rolling on activated endothelium. The interaction of P-selectin with PSGL-1 initiates the signaling pathways that lead to firm adhesion and extravasation of leukocytes. In the coagulation pathway P-selectin enhances interactions between platelets and leukocytes which support thrombus formation. Its regulation and activity are tightly linked with other proteins such as E-selectin and L-selectin in coordinating vascular and systemic inflammatory responses.

Altered expression of CD62P is associated with various inflammatory conditions and cardiovascular diseases. In thrombosis increased levels of P-selectin enhance platelet aggregation and stability of the thrombus contributing to pathological clot formation. Similarly in atherosclerosis P-selectin facilitates the recruitment of monocytes to the vessel wall promoting plaque development. The protein's function relates to interactions with key inflammatory mediators and its dysregulation is often observed in conditions like chronic inflammatory diseases and acute myocardial infarction.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B : ITGB3) and integrin alpha-V/beta-3 (ITGAV : ITGB3) (PubMed : 37184585).

See full target information SELP

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 15:1440398 PubMed39534605

2024

YTHDC1 Regulates the Migration, Invasion, Proliferation, and Apoptosis of Rheumatoid Fibroblast-Like Synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Wei Feng,Chen-Fei Yang,He-Fang Xiao,Fa Yuan,Feng Chen,Bo Zhang,Jun Zhang,Min Tan,Ming-Gang Guo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD62P antibody [EPR22850-206] - BSA and Azide free

Flow Cytometry - Anti-CD62P antibody [EPR22850-206] - BSA and Azide free (AB259996)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise