AB199000

Anti-CD68 antibody [KP1 + C68/684]

Lab Essentials
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(6 제품이 사용된 논문 )

Mouse Multiclonal CD68 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing CD68 protein.

대체 명칭 보기

CD68, Macrosialin, Gp110

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (AB199000)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (AB199000)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human histiocytoma tissue labelling CD68 with ab199000 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (AB199000)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab199000 at 1μg/ml (10X).

주요 정보

Host species

Mouse

Clonality

Multiclonal

Clone number

KP1 + C68/684

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing CD68 protein. The exact immunogen used to generate this antibody is proprietary information.

P34810

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대체품

Primary Antibodies

AB53444

Anti-CD68 antibody [FA-11]

Lab Essentials|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

Primary Antibodies

AB222914

Alexa Fluor® 488 Anti-CD68 antibody [KP1]

Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD68 antibody [KP1] (AB222914)

Primary Antibodies

AB277938

Alexa Fluor® 594 Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR20545] (AB277938)

Primary Antibodies

AB224029

Alexa Fluor® 647 Anti-CD68 antibody [EPR20545]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] (AB224029)

Primary Antibodies

AB201844

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (AB201844)

Primary Antibodies

AB201845

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (AB201845)

Primary Antibodies

AB227458

Anti-CD68 antibody [EPR20545] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458)

Primary Antibodies

AB213363

Anti-CD68 antibody [EPR20545]

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] (AB213363)

보완 제품

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information CD68

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 30:4068-4076 PubMed39042445

2024

Mutant IDH Modulates Suppressive Myeloid Populations in Malignant Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Eric P Grewal,Leland G K Richardson,Jing Sun,Rishab Ramapriyan,Maria Martinez-Lage,Julie J Miller,Bob S Carter,Daniel P Cahill,William T Curry,Bryan D Choi

Frontiers in immunology 13:936164 PubMed35990658

2022

Mice with humanized immune system as novel models to study HIV-associated pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie J Rodriguez-Irizarry,Alina C Schneider,Daniel Ahle,Justin M Smith,Edu B Suarez-Martinez,Ethan A Salazar,Brianyell McDaniel Mims,Fahmida Rasha,Hanna Moussa,Naima Moustaïd-Moussa,Kevin Pruitt,Marcelo Fonseca,Mauricio Henriquez,Matthias A Clauss,Matthew B Grisham,Sharilyn Almodovar

iScience 25:103971 PubMed35224470

2022

Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Prasanti Kotagiri,Paul A Lyons,Rafia S Al-Lamki,Federica Mescia,Laura Bergamaschi,Lorinda Turner,Michael D Morgan,Fernando J Calero-Nieto,Karsten Bach,Nicole Mende,Nicola K Wilson,Emily R Watts,Patrick H Maxwell,Patrick F Chinnery,Nathalie Kingston,Sofia Papadia,Kathleen E Stirrups,Neil Walker,Ravindra K Gupta,David K Menon,Kieren Allinson,Sarah J Aitken,Mark Toshner,Michael P Weekes,James A Nathan,Sarah R Walmsley,Willem H Ouwehand,Mary Kasanicki,Berthold Göttgens,John C Marioni,Kenneth G C Smith,Jordan S Pober,John R Bradley

Cancer immunology, immunotherapy : CII 68:1501-1513 PubMed31489465

2019

Tumor-induced peripheral immunosuppression promotes brain metastasis in patients with non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping D Li,Jonathan B Lamano,Jason B Lamano,Jessica Quaggin-Smith,Dorina Veliceasa,Gurvinder Kaur,Dauren Biyashev,Dusten Unruh,Orin Bloch

Cancer immunology, immunotherapy : CII 68:999-1009 PubMed31030234

2019

Systemic and local immunosuppression in patients with high-grade meningiomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping D Li,Dorina Veliceasa,Jason B Lamano,Jonathan B Lamano,Gurvinder Kaur,Dauren Biyashev,Craig M Horbinski,Tim J Kruser,Orin Bloch

Clinical cancer research : an official journal of 25:3643-3657 PubMed30824583

2019

Glioblastoma-Derived IL6 Induces Immunosuppressive Peripheral Myeloid Cell PD-L1 and Promotes Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan B Lamano,Jason Balquidera Lamano,Yuping D Li,Joseph D DiDomenico,Winward Choy,Dorina Veliceasa,Daniel E Oyon,Shayan Fakurnejad,Leonel Ampie,Kartik Kesavabhotla,Rajwant Kaur,Gurvinder Kaur,Dauren Biyashev,Dusten J Unruh,Craig M Horbinski,C David James,Andrew T Parsa,Orin Bloch

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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