Anti-CD7 antibody [EPR22065] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] - BSA and Azide free (AB239755)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

PDS only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : Raji (PMID : 16332967).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224194).

• Flow Cyt

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Flow Cytometry - Anti-CD7 antibody [EPR22065] - BSA and Azide free (AB239755)

Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor^® 647-conjugated anti-human CD3 and rabbit IgG - ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight^® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID : 3260936)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224194).

• IP

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Immunoprecipitation - Anti-CD7 antibody [EPR22065] - BSA and Azide free (AB239755)

CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil lysate 10 μg (Input).
Lane 2 : ab224194 IP in human tonsil lysate lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab224194 in human tonsil lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224194).

All lanes:

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (<a href='/ko/products/primary-antibodies/cd7-antibody-epr22065-ab224194'>ab224194</a>)

Predicted band size: 25 kDa

Observed band size: 37 kDa

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