JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB243003

Anti-CD70 antibody [EPR22014-176] - BSA and Azide free (Capture)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal CD70 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
1 이미지
Sandwich ELISA - Anti-CD70 antibody [EPR22014-176] - BSA and Azide free (Capture) (AB243003)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD70 antibody [EPR22014-176] - BSA and Azide free (Capture) (AB243003)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab264621).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22014-176

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Assay Kits

AB267612

Human CD70 ELISA Kit

Sandwich ELISA - Human CD70 ELISA Kit (AB267612)

  • 0
  • 0
Sample Prep & Detection Kits

AB201797

Dylight® 350 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Dylight® 350 Conjugation Kit (Fast) - Lightning-Link® (AB201797)

  • 0
  • 0
Sample Prep & Detection Kits

AB269894

Latex Conjugation Kit - 400nm Black

Functional Studies - Latex Conjugation Kit - 400nm Black (AB269894)

  • 0
  • 0
Primary Antibodies

AB269393

Anti-CD200 / OX2 antibody [EPR22412-54] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CD200 / OX2 antibody [EPR22412-54] - BSA and Azide free (Detector) (AB269393)

  • 0
  • 0
Primary Antibodies

AB285002

Anti-IL37 antibody [EPR24011-134] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL37 antibody [EPR24011-134] - BSA and Azide free (Capture) (AB285002)

  • 0
  • 0
Primary Antibodies

AB284998

Anti-IL-32 antibody [EPR23983-43] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-32 antibody [EPR23983-43] - BSA and Azide free (Capture) (AB284998)

  • 0
  • 0
Primary Antibodies

AB259726

Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] - BSA and Azide free (Detector) (AB259726)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab243003 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD70. The recommended pair for sandwich ELISA is:
Capture: ab243003, Human CD70 Capture Antibody (unconjugated)
Detector: ab242492, Human CD70 Detector Antibody (unconjugated)
The reference range value is 0.28 - 18 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
Biological function summary

CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.

Pathways

CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.

CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed : 28011863, PubMed : 28011864, PubMed : 8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed : 28011863, PubMed : 28011864).
See full target information CD70

대체 명칭 보기

CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com