Anti-CD72 antibody [EPR29739-578]

• mIHC

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Multiplex immunohistochemistry - Anti-CD72 antibody [EPR29739-578] (AB324351)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human hodgkin's lymphoma tissue staining CD72 with ab324351 at a 1 : 500 (1.018 ug/ml) dilution, ab227688 anti-CD19 used at 1 : 100 (0.15 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-CD72 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.
Panel B : anti-CD72 staining immune cells in human Hodgkin's lymphoma.
Panel C : ant-CD19 staining B lymphocytes in human Hodgkin's lymphoma.
Panel D : ant-CD3 staining T lymphocytes in human Hodgkin's lymphoma.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324351, ab227688 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] (AB324351)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD72 with ab324351 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human tonsil.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] (AB324351)

Immunohistochemical analysis of paraffin-embedded Human B-Cell follicular lymphoma tissue labeling CD72 with ab324351 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human B-Cell follicular lymphoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt

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Flow Cytometry - Anti-CD72 antibody [EPR29739-578] (AB324351)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD72 with ab324351 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD19 conjugated to Alexa Fluor® 647.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD72 antibody [EPR29739-578] (AB324351)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD72 with ab324351 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD3 conjugated to Brilliant Violet 421.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] (AB324351)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD72 with ab324351 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : Weak staining on human kidney.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins