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AB242349

Anti-CD8 alpha antibody [EPR22483-210] - BSA and Azide free (Capture/Detector)

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Rabbit Recombinant Monoclonal CD8 alpha antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-CD8 alpha antibody [EPR22483-210] - BSA and Azide free (Capture/Detector) (AB242349)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD8 alpha antibody [EPR22483-210] - BSA and Azide free (Capture/Detector) (AB242349)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab256393).

Sandwich ELISA - Anti-CD8 alpha antibody [EPR22483-210] - BSA and Azide free (Capture/Detector) (AB242349)
  • sELISA

Lab

Sandwich ELISA - Anti-CD8 alpha antibody [EPR22483-210] - BSA and Azide free (Capture/Detector) (AB242349)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab324514), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
Capture antibody : ab242349 Anti-CD8 alpha antibody [EPR22483-210] - BSA and Azide free (Capture/Detector)
Detector antibody : ab242348 Anti-CD8 alpha antibody [EPR22483-3] - BSA and Azide free (Capture/Detector)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22483-210

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB256393

Human CD8A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD8A ELISA Kit (AB256393)

  • 0
  • 0
Sample Prep & Detection Kits

AB201805

DyLight® 755 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 755 Conjugation Kit (Fast) - Lightning-Link® (AB201805)

  • 0
  • 0
Sample Prep & Detection Kits

AB188215

Gold Conjugation Kit (20 nm, 20 OD)

Conjugation - Gold Conjugation Kit (20 nm, 20 OD) (AB188215)

  • 0
  • 0
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB244668

Anti-IL-33 antibody [EPR20417-94] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-33 antibody [EPR20417-94] - BSA and Azide free (Capture) (AB244668)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB281533

Anti-Ribonuclease 3/ECP antibody [EPR23342-100] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Ribonuclease 3/ECP antibody [EPR23342-100] - BSA and Azide free (Capture) (AB281533)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242349 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD8A. The recommended pair for sandwich ELISA is:
Capture: ab242348, Human CD8A Capture Antibody (unconjugated)
Detector: ab242349, Human CD8A Detector Antibody (unconjugated)
The reference range value is 15.625 - 1000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD8A

대체 명칭 보기

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
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Product promise

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