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AB243886

Anti-CD86 antibody [BLR029F] - BSA free

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Rabbit Recombinant Monoclonal CD86 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD86.

대체 명칭 보기

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

3 이미지
Immunocytochemistry - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)
  • ICC

Unknown

Immunocytochemistry - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)

Formalin-fixed, paraffin-embedded HDLM-2 (Human Hodgkin lymphoma cell line from pleural effusion) cells labeling CD86 using ab243886 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

Immunoprecipitation - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)
  • IP

Unknown

Immunoprecipitation - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)

CD86 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243886 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243886 at 1/1000 dilution. Lane 1 : ab243886 IP in HDLM-2 whole cell lysate.

Lane 2 : Control IgG IP in HDLM-1 whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD86 antibody [BLR029F] - BSA free (ab243886)

Predicted band size: 38 kDa

false

Western blot - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)
  • WB

Unknown

Western blot - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)

All lanes:

Western blot - Anti-CD86 antibody [BLR029F] - BSA free (ab243886) at 1/1000 dilution

Lane 1:

HDLM-2 (Human Hodgkin lymphoma cell line from pleural effusion) whole cell lysate at 5 µg

Lane 2:

MJ (Human primary cutaneous T-cell non-Hodgkin lymphoma from peripheral blood) whole cell lysate at 5 µg

Lane 3:

SUP-M2 (Human ALK-positive anaplastic large cell lymphoma from cerebrospinal fluid) whole cell lysate at 50 µg

Lane 4:

KG-1 (Human acute myeloid leukemia from bone narrow) whole cell lysate at 50 µg

Lane 5:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg

Lane 6:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 7:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 8:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 9:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 10:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-rabbit IgG, HRP conjugated

Predicted band size: 38 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR029F

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human CD86. The exact immunogen used to generate this antibody is proprietary information.

P42081

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대체품

Primary Antibodies

AB234401

Anti-CD86 antibody [C86/2160R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] (AB234401)

  • 4
  • 2
Primary Antibodies

AB242020

Anti-CD86 antibody [EPR21962] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] - BSA and Azide free (AB242020)

  • 0
  • 0
Primary Antibodies

AB242142

Anti-CD86 antibody [EPR22958-106]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

  • 0
  • 0
Primary Antibodies

AB239075

Anti-CD86 antibody [EPR21962]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] (AB239075)

  • 0
  • 0
Primary Antibodies

AB263872

Anti-CD86 antibody [EPR22958-106] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (AB263872)

  • 0
  • 0

보완 제품

Assay Kits

AB242239

Human CD86 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD86 ELISA Kit (AB242239)

  • 0
  • 0
Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

  • 5
  • 17
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 6μl/0.5mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Translational cancer research 13:833-846 PubMed38482406

2024

B7-H3 promotes proliferation and migration of lung cancer cells by modulating PI3K/AKT pathway via ENO1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xianan Wu,Congcong Ding,Yingqi Liu,Ke Dong,Huizhong Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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