AB242142

Anti-CD86 antibody [EPR22958-106]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(8 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CD86 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications.

대체 명칭 보기

CD86, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, Cd86

2 이미지

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

Flow cytometric analysis of Mouse splenocytes (treated with 1 ug/ml lipopolysaccharide (LPS) for 3 days) labelling CD86 with ab242142 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab242142 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647.
Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

Flow cytometry overlay histogram showing mouse splenocytes treated with 1µg/ml LPS for 3 days (bottom) and negative untreated mouse splenocytes (top). Cells were stained with ab242142 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. The cells were incubated in 1x PBS containing 10% mouse serum and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab242142 (1x 10⁶ in 100μl at 0.2 μg/ml (1/10,500)) for 30min on ice. The cells were simultaneously stained with CD19.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/2000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22958-106

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (By similarity). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (By similarity). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (By similarity). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced (PubMed : 23241883). Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed : 23241883). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 7504059, PubMed : 7513726).

See full target information Cd86

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Materials today. Bio 33:102045 PubMed40688666

2025

Dendritic cell membrane-based DCsLipo@MnO@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Zhong,Pengcheng Wang,Jintian Wang,Yuejia Zhu,Liquan Chen,Yilong Qian,Xiang Huang,Kai Ye

IBRO neuroscience reports 18:39-49 PubMed39816480

2025

Postoperative analgesia with morphine promoting microglial activation and neuroinflammation induced by surgery aggravates perioperative neurocognitive dysfunction in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuzhi Shao,Liping Xie,Jingwen Zhai,Mingyue Ge

Frontiers in cell and developmental biology 12:1368021 PubMed38596358

2024

Deciphering perivascular macrophages and microglia in the retinal ganglion cell layers.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Yong Soo Park,Sang-Hoon Kim,Eunji Kong,Jay Kim,Jee Myung Yang,Joo Yong Lee,You-Me Kim,In-Beom Kim,Pilhan Kim

Journal of inflammation research 17:1267-1279 PubMed38434584

2024

BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei He,Danyang Jing,Shen Zhao,Meili Duan

Journal of advanced research 44:213-225 PubMed36725191

2023

Lung specific homing of diphenyleneiodonium chloride improves pulmonary fibrosis by inhibiting macrophage M2 metabolic program.

Applications

Unspecified application

Species

Unspecified reactive species

Huirui Wang,Yinghui Gao,Li Wang,Yang Yu,Jiaozhen Zhang,Chunyu Liu,Yaxin Song,Haochuan Xu,Jingcheng Wang,Hongxiang Lou,Ting Dong

Frontiers in medicine 9:897800 PubMed35911406

2022

Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP cells after spontaneous arterial recanalization.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Sang-Hoon Kim,Eunji Kong,Soo Jin Kim,Jee Myung Yang,Joo Yong Lee,Junyeop Lee,You-Me Kim,Pilhan Kim

Frontiers in pharmacology 12:719823 PubMed34744713

2021

Phillyrin Prevents Neuroinflammation-Induced Blood-Brain Barrier Damage Following Traumatic Brain Injury Altering Microglial Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jiang,Ding Wei,Xuejun He,Chao Gan,Xiaobing Long,Huaqiu Zhang

Annals of translational medicine 8:1151 PubMed33241000

2020

Dihydromyricetin attenuates neuropathic pain via enhancing the transition from M1 to M2 phenotype polarization by potentially elevating ALDH2 activity and .

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Lingxiao Yang,Longyun Li,Wei Feng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD86 antibody [EPR22958-106]

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (8)

Product promise