AB23617

Anti-CD99 antibody [DN16]

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(4 제품이 사용된 논문 )

Mouse Monoclonal CD99 antibody. Suitable for Flow Cyt, IHC-Fr and reacts with Human samples. Cited in 4 publications.

대체 명칭 보기

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

DN16

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-Fr

applications

Epitope

Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence 'LPDNENKK' located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002).

Specificity

ab23617 recognises the human CD99 cell surface antigen, a 32kD glycoprotein expressed by all leucocytes. CD99 expression tends to be highest on immature cells.

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대체품

Primary Antibodies

AB208372

Alexa Fluor® 488 Anti-CD99 antibody [EPR3096]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD99 antibody [EPR3096] (AB208372)

Primary Antibodies

AB75858

Anti-CD99 antibody [EPR3097Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

Primary Antibodies

AB226021

Anti-CD99 antibody [EPR3096] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Primary Antibodies

AB240990

Anti-CD99 antibody [SP119] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] - BSA and Azide free (AB240990)

Primary Antibodies

AB108297

Anti-CD99 antibody [EPR3096]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] (AB108297)

보완 제품

Assay Kits

AB309394

Human CD99 ELISA Kit

Sandwich ELISA - Human CD99 ELISA Kit (AB309394)

Primary Antibodies

AB281586

Anti-CD45 antibody [RM1007]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)

Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

Primary Antibodies

AB124800

Anti-Myogenin antibody [EPR4789]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (AB124800)

Primary Antibodies

AB183742

Anti-TLE 1 antibody [EPR9386(2)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-TLE 1 antibody [EPR9386(2)] (AB183742)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.

Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

See full target information CD99

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 20: PubMed30845661

2019

CD99 Expression in Glioblastoma Molecular Subtypes and Role in Migration and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Lais C Cardoso,Roseli da S Soares,Talita de S Laurentino,Antonio M Lerario,Suely K N Marie,Sueli Mieko Oba-Shinjo

Clinical cancer research : an official journal of the American Association for Cancer Research 20:678-87 PubMed24218512

2013

Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Allison F O'Neill,Jason L J Dearling,Yuchuan Wang,Tanya Tupper,Yanping Sun,Jon C Aster,Monica L Calicchio,Antonio R Perez-Atayde,Alan B Packard,Andrew L Kung

Rheumatology (Oxford, England) 48:734-40 PubMed19439502

2009

The proadhesive phenotype of systemic sclerosis skin promotes myeloid cell adhesion via ICAM-1 and VCAM-1.

Applications

ELISA, IHC-Fr

Species

Human, Human

Bradley J Rabquer,Yong Hou,Francesco Del Galdo,G Kenneth Haines,Michele L Gerber,Sergio A Jimenez,James R Seibold,Alisa E Koch

Blood 95:294-300 PubMed10607715

1999

Viral latent membrane protein 1 (LMP-1)-induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

S H Kim,Y K Shin,I S Lee,Y M Bae,H W Sohn,Y H Suh,H J Ree,M Rowe,S H Park

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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