AB212605

Anti-CD99 antibody [HO36-1.1] - BSA and Azide free

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(4 제품이 사용된 논문 )

Mouse Monoclonal CD99 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD99 protein.

대체 명칭 보기

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ewing's sarcoma tissue labeling CD99 with ab212605 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular carcinoma tissue labeling CD99 with ab212605 at 2 μg/ml.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HO36-1.1

Isotype

IgM

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing CD99 protein. The exact immunogen used to generate this antibody is proprietary information.

P14209

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대체품

Primary Antibodies

AB208372

Alexa Fluor® 488 Anti-CD99 antibody [EPR3096]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD99 antibody [EPR3096] (AB208372)

Primary Antibodies

AB75858

Anti-CD99 antibody [EPR3097Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

Primary Antibodies

AB226021

Anti-CD99 antibody [EPR3096] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Primary Antibodies

AB240990

Anti-CD99 antibody [SP119] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] - BSA and Azide free (AB240990)

Primary Antibodies

AB108297

Anti-CD99 antibody [EPR3096]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] (AB108297)

보완 제품

Assay Kits

AB309394

Human CD99 ELISA Kit

Sandwich ELISA - Human CD99 ELISA Kit (AB309394)

Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

Primary Antibodies

AB281586

Anti-CD45 antibody [RM1007]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)

Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein L

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.

Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

See full target information CD99

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of molecular biology 434:167402 PubMed34958778

2021

High-valency Anti-CD99 Antibodies Toward the Treatment of T Cell Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Larizbeth A Romero,Takamitsu Hattori,Mohamed A E Ali,Gayatri Ketavarapu,Akiko Koide,Christopher Y Park,Shohei Koide

PloS one 16:e0253170 PubMed34133426

2021

Clofarabine induces ERK/MSK/CREB activation through inhibiting CD99 on Ewing sarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Handan Sevim,Haydar Çelik,Levent Düşünceli,Ceyda S Ceyhan,Anna Molotkova,Kay Nakazawa,Garrett T Graham,Jeffrey R Petro,Jeffrey A Toretsky,Aykut Üren

Nature communications 12:2693 PubMed33976158

2021

An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Tianyu Tao,Shihua Liu,Xia Yang,Xuwei Chen,Jiaer Liang,Ruohui Hong,Wenting Wang,Yi Yang,Xiaoyi Li,Youhong Zhang,Quanfeng Li,Shujun Liang,Haocheng Yu,Yun Wu,Xinyu Guo,Yan Lai,Xiaofan Ding,Hongyu Guan,Jueheng Wu,Xun Zhu,Jie Yuan,Jun Li,Shicheng Su,Mengfeng Li,Xiuyu Cai,Junchao Cai,Han Tian

Frontiers in oncology 11:645757 PubMed33828989

2021

NSG Mice Facilitate Characterization of Ewing Sarcoma Lung Metastasis Using the PuMA Model.

Applications

Unspecified application

Species

Unspecified reactive species

Renata Scopim-Ribeiro,Michael M Lizardo,Hai-Feng Zhang,Anne-Chloé Dhez,Chistopher S Hughes,Poul H Sorensen

제품이 사용된 논문 모두 보기

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Product promise

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