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AB64877

Anti-Cdc20 antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal CDC20 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Cell division cycle protein 20 homolog.
1 이미지
Western blot - Anti-Cdc20 antibody (AB64877)
  • WB

Unknown

Western blot - Anti-Cdc20 antibody (AB64877)

All lanes:

Western blot - Anti-Cdc20 antibody (ab64877) at 1/500 dilution

Lane 1:

extract from HeLa cells, (5-30µg total protein).

Lane 2:

extract from Jurkat cells, (5-30µg total protein).

Lane 3:

extract from Jurkat cells, (5-30µg total protein) and 5-10µg of the immunising peptide.

Predicted band size: 55 kDa

Observed band size: 26 kDa,34 kDa,55 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Cell division cycle protein 20 homolog. The exact immunogen used to generate this antibody is proprietary information.

Q12834

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대체품

Primary Antibodies

AB215908

Anti-Cdc20 antibody [CDC20/1102]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [CDC20/1102] (AB215908)

  • 0
  • 0
Primary Antibodies

AB190711

Anti-Cdc20 antibody [AR12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [AR12] (AB190711)

  • 0
  • 0
Primary Antibodies

AB18217

Anti-Cdc20 antibody [BA8]

Flow Cytometry - Anti-Cdc20 antibody [BA8] (AB18217)

  • 0
  • 0
Primary Antibodies

AB185814

Anti-Cdc20 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB185814)

  • 0
  • 0
Primary Antibodies

AB183479

Anti-Cdc20 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB183479)

  • 0
  • 0
Primary Antibodies

AB86104

Anti-Cdc20 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody (AB86104)

  • 0
  • 0

보완 제품

Primary Antibodies

AB131272

Anti-ACTL6A antibody [EPR7443]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443] (AB131272)

  • 0
  • 0
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 5
  • 17
Primary Antibodies

AB252940

Anti-UBE2C antibody [EPR23165-31]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2C antibody [EPR23165-31] (AB252940)

  • 0
  • 0
Primary Antibodies

AB229912

Anti-PERK antibody [EPR19876-294]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-PERK antibody [EPR19876-294] (AB229912)

  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.
Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation (PubMed : 9734353, PubMed : 27030811, PubMed : 29343641). Recognizes and binds the destruction box (D box) on protein substrates (PubMed : 29343641). Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates (PubMed : 9811605, PubMed : 9637688). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity). The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).
See full target information Cell division cycle protein 20 homolog

대체 명칭 보기

Cell division cycle protein 20 homolog, p55CDC, CDC20
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental & molecular medicine 50:46 PubMed29700288

2018

ATP depletion during mitotic arrest induces mitotic slippage and APC/C-dependent cyclin B1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Yeon Park,Ju-Hyun Ahn,Min-Guk Cho,Jae-Ho Lee

Cell cycle (Georgetown, Tex.) 14:1459-70 PubMed25789401

2015

Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy.

Applications

WB

Species

Human

Chye Ling Tan,Sébastien Teissier,Jayantha Gunaratne,Ling Shih Quek,Sophie Bellanger

The Journal of cell biology 206:843-53 PubMed25246615

2014

Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.

Applications

Unspecified application

Species

Mouse

Deepak Adhikari,M Kasim Diril,Kiran Busayavalasa,Sanjiv Risal,Shoma Nakagawa,Rebecca Lindkvist,Yan Shen,Vincenzo Coppola,Lino Tessarollo,Nobuaki R Kudo,Philipp Kaldis,Kui Liu

Nature structural & molecular biology 19:1116-23 PubMed23007861

2012

APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.

Applications

WB

Species

Human

Kristina Uzunova,Billy T Dye,Hannelore Schutz,Rene Ladurner,Georg Petzold,Yusuke Toyoda,Marc A Jarvis,Nicholas G Brown,Ina Poser,Maria Novatchkova,Karl Mechtler,Anthony A Hyman,Holger Stark,Brenda A Schulman,Jan-Michael Peters

Cell 141:81-93 PubMed20371347

2010

Persistent telomere damage induces bypass of mitosis and tetraploidy.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Davoli,Eros Lazzerini Denchi,Titia de Lange
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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