Anti-CDCA5 antibody [EPR16331] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328)

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls : anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192237).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328)

Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5 with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192237).

• WB

Supplier Data

Western blot - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192237). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Blocking and diluting buffer and concntration : 5% NFDM/TBST Imaging carried out using iBright CL 1000 Imaging System Exposure time : Lane 1 : 180 seconds, Lane 2 : 48 seconds.

All lanes:

Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (<a href='/ko/products/primary-antibodies/cdca5-antibody-epr16331-c-terminal-ab192237'>ab192237</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 27 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328)

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192237).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240328).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.