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AB199728

Anti-Cdk4 antibody [EPR17525]

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(87 제품이 사용된 논문 )

Anti-Cdk4 antibody [EPR17525] (ab199728) is a rabbit monoclonal antibody detecting Cdk4 in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications

대체 명칭 보기

Crk3, Cdk4, Cyclin-dependent kinase 4, CRK3, Cell division protein kinase 4, PSK-J3

7 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR17525] (AB199728)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cdk4 with ab199728 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H & L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic, nuclear and membrane staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab199728 at 1/250 dilution followed by ab150120 at 1/500 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR17525] (AB199728)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Cdk4 with ab199728 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody- Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab199728 at 1/250 dilution followed by ab150120 at 1/500 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.

Immunoprecipitation - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cdk4 antibody [EPR17525] (AB199728)

Cdk4 was immunoprecipitated from 1mg NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab199728 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199728 at 1/1000 dilution. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

Lane 1 : NIH/3T3 whole cell lysate, 10 (Input).

Lane 2 : ab199728 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab199728 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-Cdk4 antibody [EPR17525] (ab199728)

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • WB

Supplier Data

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L, Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 15s

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • WB

Supplier Data

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/20000 dilution

All lanes:

C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L, Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 30s

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • WB

Unknown

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/2000 dilution

All lanes:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

true

Exposure time: 30s

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
  • WB

Supplier Data

Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/10000 dilution

Lane 1:

Mouse brain whole cell lysate at 10 µg

Lane 2:

Mouse heart whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 1min

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Cdk4 antibody [EPR17525] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17525

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>The immunogen used for this antibody shares homology with CDK6 (~57%) and with CDK3, CDK5, CDK2 and CDK1 (~40–44%). Cross-reactivity with these proteins has not been tested experimentally.</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

제품 세부 정보

What is this antibody validated in?
Anti-Cdk4 antibody [EPR17525] (ab199728) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Cdk4?
Anti-Cdk4 [EPR17525] (ab199728) specifically detects a band for Cdk4 (UniProt: P30285) at a molecular weight of 34kDa.

Trusted by the scientific community
Anti-Cdk4 [EPR17525] (ab199728) was first used in a scientific publication in 2016 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR17525] also available for your convenience: ab199728, Alexa Fluor® 647 - ab225420, Carrier free - ab236019

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cdk4 also known as Cyclin-dependent kinase 4 is a serine/threonine kinase involved in cell cycle regulation. Cdk4 has a molecular weight of approximately 34 kDa. This protein mainly locates in the nucleus of the cell and its expression occurs in diverse tissues. Cdk4 partners with D-type cyclins to exert its function which is essential for the transition from the G1 phase to the S phase of the cell cycle. Researchers often study Cdk4 using techniques like immunohistochemistry (IHC) to determine its expression and localization in different tissues.
Biological function summary

Cdk4 plays a fundamental role in cell cycle regulation by forming a complex with D-type cyclins. Together they phosphorylate the retinoblastoma protein (Rb) resulting in the release of E2F transcription factors which promote the expression of genes necessary for DNA replication. The Cdk4/cyclin D complex regulates the cell's commitment to enter S phase and continue cell division. This regulation is key for normal cell proliferation and tissue homeostasis. Cdk4 activity is strictly controlled by INK4 family members acting as inhibitors and adding an extra regulation layer.

Pathways

Cdk4 is an integral part of important signaling pathways like the cell cycle and PI3K/AKT pathways. In the cell cycle pathway Cdk4 relays signals downstream that drive the transition from the G1 to S phase through its interaction with cyclin D and Rb. In the PI3K/AKT pathway signaling can influence cyclin D expression indirectly affecting Cdk4 activity. Proteins such as Cdk6 closely relate to Cdk4 and often compensate or partner with Cdk4 in these pathways to maintain proper cell cycle progression.

Abnormalities in Cdk4 function or regulation relate to cancer and certain metabolic syndromes. Hyperactivity of Cdk4 due to overexpression or mutation frequently occurs in several cancers including melanoma and breast cancer. In these malignancies Cdk4 aberrantly drives excessive cell proliferation. Mutations or alterations in proteins such as p16 an inhibitor of Cdk4 are often found in these cancers highlighting the critical role of Cdk4 in disease progression. Understanding the role of Cdk4 allows researchers to develop targeted therapies such as Cdk4/6 inhibitors offering new treatment options.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
See full target information Cdk4

제품이 사용된 논문 (87)

Recent publications for all applications. Explore the 전체 목록 and refine your search

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Astragaloside IV Alleviates Fructose-Induced Intestinal Metabolic Senescence by Targeting Ketohexokinase Asn261/Ala226 to Preserve Intestinal Stem Cell Homeostasis.

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Raddeanin A exerts potent efficacy against non-small cell lung cancer by inhibiting cyclin-dependent kinase 6.

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Xian Wang,Xiao Lin,Yuxin Liu,Chunbo Ma,Mengchu Liu,Jiayu Bai,Yihan Ye,Chengguang Zhao,Lehe Yang,Xiaoying Huang,Liangxing Wang

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Genome biology 25:301 PubMed39617889

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Daniel J Merk,Foteini Tsiami,Sophie Hirsch,Bianca Walter,Lara A Haeusser,Jens D Maile,Aaron Stahl,Mohamed A Jarboui,Anna Lechado-Terradas,Franziska Klose,Sepideh Babaei,Jakob Admard,Nicolas Casadei,Cristiana Roggia,Michael Spohn,Jens Schittenhelm,Stephan Singer,Ulrich Schüller,Federica Piccioni,Nicole S Persky,Manfred Claassen,Marcos Tatagiba,Philipp J Kahle,David E Root,Markus Templin,Ghazaleh Tabatabai

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SPINK13 acts as a tumor suppressor in hepatocellular carcinoma by inhibiting Akt phosphorylation.

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METTL3-mediated m6A modification increases Hspa1a stability to inhibit osteoblast aging.

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Yaobin Wang,Yi Chen,Hefang Xiao,Zhongcheng Liu,Xuening Liu,Zhiwei Feng,Xiaoyun Sheng,Bo Peng,Xiaojun Ren,Lihu Xu,Fei Teng,Zhi Yi,YongKang Niu,Dejian Xiang,Yayi Xia,Bin Geng

Cell chemical biology 31:1162-1175.e10 PubMed38320555

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The cyclimids: Degron-inspired cereblon binders for targeted protein degradation.

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Species

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Saki Ichikawa,N Connor Payne,Wenqing Xu,Chia-Fu Chang,Nandini Vallavoju,Spencer Frome,Hope A Flaxman,Ralph Mazitschek,Christina M Woo

Cardiovascular research 120:152-163 PubMed38175760

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Gene therapy encoding cell cycle factors to treat chronic ischemic heart failure in rats.

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Species

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Riham R E Abouleisa,Xian-Liang Tang,Qinghui Ou,Abou-Bakr M Salama,Amie Woolard,Dana Hammouri,Hania Abdelhafez,Sarah Cayton,Sameeha K Abdulwali,Momo Arai,Israel D Sithu,Daniel J Conklin,Roberto Bolli,Tamer M A Mohamed

Communications biology 6:1041 PubMed37833461

2023

DNA damage induced by CDK4 and CDK6 blockade triggers anti-tumor immune responses through cGAS-STING pathway.

Applications

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Species

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Huimin Fan,Wancheng Liu,Yanqiong Zeng,Ying Zhou,Meiling Gao,Liping Yang,Hao Liu,Yueyue Shi,Lili Li,Jiayuan Ma,Jiayin Ruan,Ruyun Cao,Xiaoxia Jin,Jian Chen,Genhong Cheng,Heng Yang
제품이 사용된 논문 모두 보기

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