Anti-Cdk4 antibody [EPR17525]
- 20ul selling size
- RabMAb
- Recombinant
- 각 태그의 뜻
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(87 제품이 사용된 논문 )
Anti-Cdk4 antibody [EPR17525] (ab199728) is a rabbit monoclonal antibody detecting Cdk4 in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications
대체 명칭 보기
Crk3, Cdk4, Cyclin-dependent kinase 4, CRK3, Cell division protein kinase 4, PSK-J3
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR17525] (AB199728)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cdk4 with ab199728 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H & L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic, nuclear and membrane staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab199728 at 1/250 dilution followed by ab150120 at 1/500 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR17525] (AB199728)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Cdk4 with ab199728 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody- Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab199728 at 1/250 dilution followed by ab150120 at 1/500 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-Cdk4 antibody [EPR17525] (AB199728)
Cdk4 was immunoprecipitated from 1mg NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab199728 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199728 at 1/1000 dilution. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1 : NIH/3T3 whole cell lysate, 10 (Input).
Lane 2 : ab199728 IP in NIH/3T3 whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab199728 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds
All lanes:
Immunoprecipitation - Anti-Cdk4 antibody [EPR17525] (ab199728)
Predicted band size: 34 kDa
Observed band size: 34 kDa
false
- WB
Supplier Data
Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/2000 dilution
Lane 1:
C6 (Rat glial tumor cell line) at 10 µg
Lane 2:
PC-12 (Rat adrenal gland pheochromocytoma cell line) at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L, Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
true
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/20000 dilution
All lanes:
C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L, Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
true
Exposure time: 30s
- WB
Unknown
Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/2000 dilution
All lanes:
NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
true
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-Cdk4 antibody [EPR17525] (AB199728)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Cdk4 antibody [EPR17525] (ab199728) at 1/10000 dilution
Lane 1:
Mouse brain whole cell lysate at 10 µg
Lane 2:
Mouse heart whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
true
Exposure time: 1min
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-Cdk4 antibody [EPR17525] - BSA and Azide free
Reactivity 정보
제품 세부 정보
What is this antibody validated in?
Anti-Cdk4 antibody [EPR17525] (ab199728) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Cdk4?
Anti-Cdk4 [EPR17525] (ab199728) specifically detects a band for Cdk4 (UniProt: P30285) at a molecular weight of 34kDa.
Trusted by the scientific community
Anti-Cdk4 [EPR17525] (ab199728) was first used in a scientific publication in 2016 and has been cited over 60 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR17525] also available for your convenience: ab199728, Alexa Fluor® 647 - ab225420, Carrier free - ab236019
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cdk4 plays a fundamental role in cell cycle regulation by forming a complex with D-type cyclins. Together they phosphorylate the retinoblastoma protein (Rb) resulting in the release of E2F transcription factors which promote the expression of genes necessary for DNA replication. The Cdk4/cyclin D complex regulates the cell's commitment to enter S phase and continue cell division. This regulation is key for normal cell proliferation and tissue homeostasis. Cdk4 activity is strictly controlled by INK4 family members acting as inhibitors and adding an extra regulation layer.
Pathways
Cdk4 is an integral part of important signaling pathways like the cell cycle and PI3K/AKT pathways. In the cell cycle pathway Cdk4 relays signals downstream that drive the transition from the G1 to S phase through its interaction with cyclin D and Rb. In the PI3K/AKT pathway signaling can influence cyclin D expression indirectly affecting Cdk4 activity. Proteins such as Cdk6 closely relate to Cdk4 and often compensate or partner with Cdk4 in these pathways to maintain proper cell cycle progression.
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타겟 정보
제품이 사용된 논문 (87)
Recent publications for all applications. Explore the 전체 목록 and refine your search
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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