Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (AB242399)

Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (AB242399)

Intracellular Flow Cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green).

This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (AB242399)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (AB242399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

• IP

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Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (AB242399)

Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

All lanes:

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (<a href='/ko/products/primary-antibodies/cdk5rap3-antibody-epr11294b-ab157203'>ab157203</a>)

Predicted band size: 57 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (AB242399)

ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab157203 & HEK-293 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

All lanes:

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (<a href='/ko/products/primary-antibodies/cdk5rap3-antibody-epr11294b-ab157203'>ab157203</a>)

Predicted band size: 57 kDa

false