Anti-CDKL5 antibody

• WB

Collaborator

Western blot - Anti-CDKL5 antibody (AB22453)

Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID : 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.

All lanes:

Western blot - Anti-CDKL5 antibody (ab22453) at 3 µg/mL

Lane 1:

mouse cortex at 20 µg

Lane 2:

mouse cortex at 20 µg with Human CDKL5 peptide (<a href='/ko/products/unavailable/human-cdkl5-peptide-ab25756'>ab25756</a>)

Predicted band size: 116 kDa

Observed band size: 120 kDa,200 kDa

false

Exposure time: 10min

This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom