AB241543

Anti-CDKN2A/p16INK4a antibody [PABLO33B]

Name: Anti-CDKN2A/p16INK4a antibody [PABLO33B]
Brand: Abcam Limited
SKU: AB241543
Rating: 5 (1 reviews)

Recombinant
KO Validated
각 태그의 뜻

(1 리뷰)

(19 제품이 사용된 논문 )

Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543) is a rat monoclonal antibody detecting CDKN2A/p16INK4a in IHC-P. Suitable for Mouse.

- KO validated for confirmed specificity

대체 명칭 보기

P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (AB241543)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (AB241543)

Immunohistochemical analysis of paraffin-embedded Wild type mouse fibrosarcoma and p16 knock-out mouse fibrosarcoma tissue labeling CDKN2A/p16INK4a with ab241543 at 1/640 dilution (1.28 μg/mL).

Image A : Positive staining on the wild type mouse fibrosarcoma

Image B : No staining on the p16 knock-out mouse fibrosarcoma

The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.

The image was kindly provided by Spanish Nacional Cancer Research Centre (CNIO)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (AB241543)

Immunohistochemical analysis of paraffin-embedded mouse papilloma tissue labeling CDKN2A/p16INK4a with ab241543 at 1/640 dilution (1.28 μg/mL). Positive staining on the mouse papilloma. The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.

The image was kindly provided by Spanish Nacional Cancer Research Centre (CNIO)

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

PABLO33B

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/640", "IHCP-species-notes": "<p></p>" } } }

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제품 세부 정보

Product Specifications

Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543) is a rat recombinant monoclonal antibody and is validated for use in in mouse samples.
Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543) specifically detects CDKN2A/p16INK4a (UniProt ID: P51480; Molecular weight: 18kDa) and is sold in a convenient trial size to enable initial testing (20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543) has been confirmed by testing in knockout samples.

Related Products

Conjugation-ready, carrier free format available for antibody clone PABLO33B - ab252788.

Target Information

CDKN2A, also known as cyclin-dependent kinase inhibitor 2A, is a crucial tumor suppressor gene located on chromosome 9p211. It encodes two proteins, p16INK4a and p14ARF, both of which play significant roles in regulating the cell cycle. p16INK4a inhibits cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), preventing the progression from the G1 to S phase of the cell cycle. This inhibition is vital for controlling cell proliferation and preventing tumor growth. Mutations or deletions in CDKN2A are frequently associated with various cancers, including melanoma, pancreatic cancer, and head and neck squamous cell carcinoma.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Ion exchange chromatography

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

See full target information Cdkn2a

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제품이 사용된 논문 (19)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nano-micro letters 18:11 PubMed40699546

2025

A Hierarchical Short Microneedle-Cupping Dual-Amplified Patch Enables Accelerated, Uniform, Pain-Free Transdermal Delivery of Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Minwoo Song,Minji Ha,Sol Shin,Minjin Kim,Soyoung Son,Jihyun Lee,Gui Won Hwang,Jeongyun Kim,Van Hieu Duong,Jae Hyung Park,Changhyun Pang

Frontiers in aging 6:1583288 PubMed40535023

2025

Membrane transporter progressive ankylosis protein homologue (/) partially mediates senescence-derived extracellular citrate and is regulated by DNA damage, inflammation, and ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Naomi James,Muy-Teck Teh,Yufeng Li,Christine Wagner-Bock,Zahra Falah Al-Khateeb,Lee Peng Karen-Ng,Terry Roberts,Linnea Synchyshyn,Amy Lewis,Ana O'Loghlen,Andrew Silver,Adina Teodora Michael-Titus,Mark Bennett,Jacob Guy Bundy,Maria Elzbieta Mycielska,Eric Kenneth Parkinson

Frontiers in pharmacology 16:1506518 PubMed40385483

2025

Benzyl isothiocyanate provokes senolysis by targeting AKT in senescent IPF fibroblasts and reverses persistent pulmonary fibrosis in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Fan Yang,Ying Liu,Meiting Peng,Lu Yu,Qinhui Hou,Yuan Liu,Zhenshun Cheng

Frontiers in pharmacology 16:1562321 PubMed40110129

2025

Sodium nitrate regulates senescence accompanied by aortic atherosclerosis in ApoE mice through the miR-34a/FGF-21 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Tao,Zhichao He,Han Duan,Liang Wang,Jing Yi,Jingyuan Shao,Lin Lv,Junzhao Duan,Hu Cao,Xiwen Dong,Hua Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400502 PubMed39418070

2024

Insufficient Mechanical Loading Downregulates Piezo1 in Chondrocytes and Impairs Fracture Healing Through ApoE-Induced Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Siming Jia,Weijian Liu,Mo Zhang,Lijun Wang,Chuan Ren,Chen Feng,Tao Zhang,Hongzhi Lv,Zhiyong Hou,Weiguo Zou,Yingze Zhang,Wei Tong,Juan Wang,Wei Chen

Histology and histopathology 40:733-743 PubMed39380528

2024

Mechanism of KMT2D-mediated epigenetic modification in IL-1β-induced nucleus pulposus cell degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjiang Liu,Haiquan Liu,Zuyu Meng,Wensheng Zhang

Science advances 10:eadn1607 PubMed39110807

2024

Cell-specific cross-talk proteomics reveals cathepsin B signaling as a driver of glioblastoma malignancy near the subventricular zone.

Applications

Unspecified application

Species

Unspecified reactive species

Emily S Norton,Lauren A Whaley,Vanessa K Jones,Mieu M Brooks,Marissa N Russo,Dmytro Morderer,Erik Jessen,Paula Schiapparelli,Andres Ramos-Fresnedo,Natanael Zarco,Anna Carrano,Wilfried Rossoll,Yan W Asmann,TuKiet T Lam,Kaisorn L Chaichana,Panos Z Anastasiadis,Alfredo Quiñones-Hinojosa,Hugo Guerrero-Cázares

Human genomics 18:55 PubMed38822443

2024

Pan-cancer analysis of CDKN2A alterations identifies a subset of gastric cancer with a cold tumor immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Deng,Zi-Xi Li,Chen-Jun Xie,Qing-Lin Zhang,Ben-Shun Hu,Mei-Dan Wang,Jie Mei,Chen Yang,Zhangfeng Zhong,Ke-Wei Wang

Molecular biology reports 51:385 PubMed38438773

2024

CDKN2A promoter methylation enhances self-renewal of glioblastoma stem cells and confers resistance to carmustine.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Yan-Feng Xi,Qi Zhao,Jiang-Hong Guo,Zhen Zhang,Mao-Bai Zhang,Jiang Chang,Yue-Qin Wu,Wen Su

iScience 27:108721 PubMed38303704

2024

n-3 polyunsaturated fatty acids delay intervertebral disc degeneration by inhibiting nuclear receptor coactivator 4-mediated iron overload.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ao,Tao Jiang,Yuan Li,Weiyi Lai,Zhengnan Lian,Liang Wang,Minjun Huang,Zhongmin Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDKN2A/p16INK4a antibody [PABLO33B]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (AB241543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (AB241543)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (19)

Product promise