AB174939

Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free

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(2 제품이 사용된 논문 )

Rat Monoclonal p16INK4A antibody. Carrier free. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 2 publications.

대체 명칭 보기

P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (AB174939)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (AB174939)

This ICC/IF data was generated using the same antibody clone in a different buffer formulation (ab26696).

Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

5-C3

Isotype

IgG2b

Carrier free

Yes

Reacts with

Mouse

Applications

ICC/IF, WB, IHC-FoFr, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab174939 is the carrier-free version of ab26696.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purity

IgG fraction

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

See full target information Cdkn2a

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 41:111596 PubMed36351380

2022

Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fraser R Millar,Adam Pennycuick,Morwenna Muir,Andrea Quintanilla,Priya Hari,Elisabeth Freyer,Philippe Gautier,Alison Meynert,Graeme Grimes,Carla Salomo Coll,Sofia Zdral,Stella Victorelli,Vitor H Teixeira,John Connelly,João F Passos,Marian A Ros,William A H Wallace,Margaret C Frame,Andrew H Sims,Luke Boulter,Sam M Janes,Simon Wilkinson,Juan Carlos Acosta

Journal of neurochemistry 134:327-39 PubMed25865831

2015

Drebrin depletion alters neurotransmitter receptor levels in protein complexes, dendritic spine morphogenesis and memory-related synaptic plasticity in the mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Gangsoo Jung,Eun-Jung Kim,Ana Cicvaric,Sunetra Sase,Marion Gröger,Harald Höger,Fernando Jayson Sialana,Johannes Berger,Francisco J Monje,Gert Lubec

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (AB174939)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise