Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721]
Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 항체 [EPR20721]
- BOND RX™ Validated
- RabMAb
- Recombinant
- 20ul selling size
- 각 태그의 뜻
Be the first to review this product! 리뷰 제출
|
(12 제품이 사용된 논문 )
Rabbit Recombinant Monoclonal CEACAM5 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 12 publications.
대체 명칭 보기
CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5, CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6, CD66a, BGP, BGP1, CEACAM1, Cell adhesion molecule CEACAM1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP-1, CEA cell adhesion molecule 1, CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon cancer (PMID : 25042385) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Immunohistochemistry analysis of paraffin-embedded (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag, (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag, (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag, (F) HEK-293T cells transfected with empty vector containing a his tag, sections labelling CEACAM5 + CD66b + CEACAM1 + CEACAM6 with ab207718 at 1/5000 dilution. The section was incubated with ab207718 for 30 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive stainng on (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag, (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag. Nearly no staining on (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag and (F) HEK-293T cells transfected with empty vector containing a his tag. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of spleen-infiltrating neutrophils (PMID : 22064717 and 19077207) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Carcino Embryonic Antigen CEA with ab207718 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membranous staining on MCF7 cell line.
Negative control for cells : PANC-1 .
Counterstained with ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and DAPI (blue).
The negative control is the secondary antibody only.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Flow cytometric analysis of PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) (Left) / MCF7 (human breast adenocarcinoma epithelial cell) (Right) labeling Carcino Embryonic Antigen CEA with ab207718 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were not fixed or permeabilized. Gated on total viable cells.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon (PMID : 25042385) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- WB
Supplier Data
Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.
Exposure times :
Lanes 1-3 : 10 seconds
Lane 4 : 4 minutes
Observed MW : 50, 30, 32, 75 kDa.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
All lanes:
Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (ab207718) at 1/1000 dilution
Lane 1:
His-GST-tagged human CEACAM5 recombinant protein (aa238-686) 10 ng
Lane 2:
His-tagged human CEACAM6 recombinant protein (aa35-326) 10 ng
Lane 3:
His-tagged human CEACAM8 recombinant protein (aa35-335) 10 ng
Lane 4:
His-tagged human CEACAM1 recombinant protein (aa35-428) 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 30 kDa,32 kDa,50 kDa,75 kDa
true
- WB
Supplier Data
Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.
Observed MW : 110-150, 75-90 kDa.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
All lanes:
Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (ab207718) at 1/1000 dilution
Lane 1:
MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2:
PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 110-150 kDa,75-90 kDa
true
Exposure time: 58s
- WB
Supplier Data
Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.
Exposure times :
Lanes 1-2 : 3 minutes
Lanes 3-4 : 10 seconds
Observed MW 110-150, 75-90 kDa.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The expression profile observed is consistent with differential glycosylation of this protein (PMID : 1717152).
All lanes:
Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (ab207718) at 1/1000 dilution
Lane 1:
Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2:
SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 3:
Human colon lysate at 20 µg
Lane 4:
LoVo (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 110-150 kDa,75-90 kDa
true
관련 conjugated 항체와 다양한 조성의 항체 (1)
-
Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free
Reactivity 정보
제품 세부 정보
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CEACAM5 proteins influence cell signaling and intercellular communication. They participate in immune response modulation and function as part of larger protein complexes. CEACAM5 acts as an anchor point for various cellular processes by associating with CEACAM1 and CEACAM6. By regulating cell adhesion CEACAM5 helps in maintaining normal cellular architecture and function within tissue environments.
Pathways
CEACAM5 is integral to pathways involving cell adhesion and signaling. It participates in immune system responses and the regulation of cell proliferation pathways including the MEK/ERK signaling cascade. CEACAM5 proteins interact with others like CEACAM1 sharing roles in cell signaling and communication networks that control adhesion dynamics and growth factor responses.
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
Additional targets
제품이 사용된 논문 (12)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Cell reports. Medicine 5:101627 PubMed38964315
2024
Applications
Unspecified application
Species
Unspecified reactive species
NPJ precision oncology 8:109 PubMed38769374
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature biomedical engineering 8:345-360 PubMed38114742
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cell communication and signaling : CCS 21:176 PubMed37480055
2023
Applications
Unspecified application
Species
Unspecified reactive species
World journal of surgical oncology 20:266 PubMed36008864
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cancer cell international 22:96 PubMed35193596
2022
Applications
Unspecified application
Species
Unspecified reactive species
Bioengineered 13:5525-5536 PubMed35166644
2022
Applications
Unspecified application
Species
Unspecified reactive species
World journal of surgical oncology 20:32 PubMed35135563
2022
Applications
Unspecified application
Species
Unspecified reactive species
JBMR plus 6:e10596 PubMed35309866
2022
Applications
Unspecified application
Species
Unspecified reactive species
Clinics (Sao Paulo, Brazil) 76:e3318 PubMed34817046
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com