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AB207718

Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721]

Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 항체 [EPR20721]

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(12 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CEACAM5 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 12 publications.

대체 명칭 보기

CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5, CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6, CD66a, BGP, BGP1, CEACAM1, Cell adhesion molecule CEACAM1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP-1, CEA cell adhesion molecule 1, CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8

9 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon cancer (PMID : 25042385) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Immunohistochemistry analysis of paraffin-embedded (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag, (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag, (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag, (F) HEK-293T cells transfected with empty vector containing a his tag, sections labelling CEACAM5 + CD66b + CEACAM1 + CEACAM6 with ab207718 at 1/5000 dilution. The section was incubated with ab207718 for 30 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive stainng on (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag, (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag. Nearly no staining on (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag and (F) HEK-293T cells transfected with empty vector containing a his tag. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of spleen-infiltrating neutrophils (PMID : 22064717 and 19077207) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Carcino Embryonic Antigen CEA with ab207718 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membranous staining on MCF7 cell line.

Negative control for cells : PANC-1 .

Counterstained with ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and DAPI (blue).

The negative control is the secondary antibody only.

Flow Cytometry - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Flow cytometric analysis of PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) (Left) / MCF7 (human breast adenocarcinoma epithelial cell) (Right) labeling Carcino Embryonic Antigen CEA with ab207718 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were not fixed or permeabilized. Gated on total viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon (PMID : 25042385) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • WB

Supplier Data

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

Exposure times :

Lanes 1-3 : 10 seconds

Lane 4 : 4 minutes

Observed MW : 50, 30, 32, 75 kDa.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (ab207718) at 1/1000 dilution

Lane 1:

His-GST-tagged human CEACAM5 recombinant protein (aa238-686) 10 ng

Lane 2:

His-tagged human CEACAM6 recombinant protein (aa35-326) 10 ng

Lane 3:

His-tagged human CEACAM8 recombinant protein (aa35-335) 10 ng

Lane 4:

His-tagged human CEACAM1 recombinant protein (aa35-428) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,32 kDa,50 kDa,75 kDa

true

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • WB

Supplier Data

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

Observed MW : 110-150, 75-90 kDa.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (ab207718) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-150 kDa,75-90 kDa

true

Exposure time: 58s

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)
  • WB

Supplier Data

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (AB207718)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

Exposure times :

Lanes 1-2 : 3 minutes

Lanes 3-4 : 10 seconds

Observed MW 110-150, 75-90 kDa.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The expression profile observed is consistent with differential glycosylation of this protein (PMID : 1717152).

All lanes:

Western blot - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] (ab207718) at 1/1000 dilution

Lane 1:

Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

Human colon lysate at 20 µg

Lane 4:

LoVo (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-150 kDa,75-90 kDa

true

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20721

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab207718 cross-reacts with CEACAM 1, 6 and 8&#46; There are other CEACAM family members but we only examined cross-reactivity with those having &gt;55% homology with CEACAM5 following a BLAST search with the immunogen.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/40000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/40000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM5 also known as CEA or CD66e is part of the carcinoembryonic antigen family. It plays a role as a cell adhesion molecule facilitating intercellular binding. CEACAM5 is a glycoprotein with a molecular mass of approximately 180 kDa. You find CEACAM5 expression in normal epithelial tissues such as the colon stomach and tongue but it shows increased levels in various carcinoma cells. It is also identified as CEACAM6 and both of these linked proteins function as cell surface glycoproteins involved in cellular interactions.
Biological function summary

CEACAM5 proteins influence cell signaling and intercellular communication. They participate in immune response modulation and function as part of larger protein complexes. CEACAM5 acts as an anchor point for various cellular processes by associating with CEACAM1 and CEACAM6. By regulating cell adhesion CEACAM5 helps in maintaining normal cellular architecture and function within tissue environments.

Pathways

CEACAM5 is integral to pathways involving cell adhesion and signaling. It participates in immune system responses and the regulation of cell proliferation pathways including the MEK/ERK signaling cascade. CEACAM5 proteins interact with others like CEACAM1 sharing roles in cell signaling and communication networks that control adhesion dynamics and growth factor responses.

Excessive CEACAM5 expression links to colorectal and gastric cancers making it a useful cancer biomarker. CEACAM5-positive tumors often correlate with disease progression which highlights their potential as therapeutic targets. These proteins also interact with CD66b markers which are associated with neutrophil activation in inflammatory responses. Such associations with CEACAM1 and CEACAM6 in carcinogenic pathways further underline CEACAM5's relevance in cancer diagnostics and treatment strategies.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed : 10864933, PubMed : 10910050, PubMed : 2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed : 10910050).. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
See full target information CEACAM5

Additional targets

CEACAM1,CEACAM6,CEACAM8

제품이 사용된 논문 (12)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports. Medicine 5:101627 PubMed38964315

2024

Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Shangru Li,Lefan Zhu,Mingle Huang,Yubin Xie,Xinming Song,Zhihui Chen,Harry Cheuk-Hay Lau,Joseph Jao-Yiu Sung,Lixia Xu,Jun Yu,Xiaoxing Li

NPJ precision oncology 8:109 PubMed38769374

2024

Multiomics integration reveals NETosis heterogeneity and TLR2 as a prognostic biomarker in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Fu,Jinxin Tao,Yani Gu,Yueze Liu,Jiangdong Qiu,Dan Su,Ruobing Wang,Wenhao Luo,Tao Liu,Feifan Zhang,Taiping Zhang,Yupei Zhao

Nature biomedical engineering 8:345-360 PubMed38114742

2023

Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids.

Applications

Unspecified application

Species

Unspecified reactive species

Marius F Harter,Timothy Recaldin,Regine Gerard,Blandine Avignon,Yannik Bollen,Cinzia Esposito,Karolina Guja-Jarosz,Kristina Kromer,Adrian Filip,Julien Aubert,Anneliese Schneider,Marina Bacac,Michael Bscheider,Nadine Stokar-Regenscheit,Salvatore Piscuoglio,Joep Beumer,Nikolche Gjorevski

Cell communication and signaling : CCS 21:176 PubMed37480055

2023

Neutrophil extracellular traps promote angiogenesis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shifeng Yang,Boshi Sun,Jiacheng Li,Nana Li,Ange Zhang,Xinyu Zhang,Hao Yang,Xiaoming Zou

World journal of surgical oncology 20:266 PubMed36008864

2022

Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Enqi Zheng,Zhun Cai,Wangyong Li,Chuandou Ni,Qian Fang

Cancer cell international 22:96 PubMed35193596

2022

miR-223-3p carried by cancer-associated fibroblast microvesicles targets SORBS1 to modulate the progression of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Jin,Xi Qiu,Yi Huang,Hang Zhang,Kaibo Chen

Bioengineered 13:5525-5536 PubMed35166644

2022

The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Hui Ma,Lisha Ma,Kun Li,Yanhua Kang

World journal of surgical oncology 20:32 PubMed35135563

2022

Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Longhua Feng,Pengjiang Cheng,Zhengyun Feng,Xiaoyu Zhang

JBMR plus 6:e10596 PubMed35309866

2022

A Subset of Osteosarcoma Bears Markers of CXCL12-Abundant Reticular Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Branden R Sosa,Ziqi Wang,John H Healey,Meera Hameed,Matthew B Greenblatt

Clinics (Sao Paulo, Brazil) 76:e3318 PubMed34817046

2021

RNA-binding protein with serine-rich domain 1 regulates microsatellite instability of uterine corpus endometrial adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Hui Ma,Lisha Ma,Kun Li,Yanhua Kang
제품이 사용된 논문 모두 보기

Product promise

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